消痔灵注射液促进血管瘤内皮细胞凋亡作用的研究  

Study of Xiaozhiling injection in hemangioma endothelial cell promote apoptosis tumor

作　　者：李善昌[1] 梁显峰 姜炳华[1] 闫磊[1] 宁尚波[1] 

机构地区：[1]佳木斯大学附属第二医院口腔颌面外科,黑龙江佳木斯154002

出　　处：《黑龙江医药科学》2015年第4期40-42,共3页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学研究生科技创新项目;编号:LM2014-044

摘　　要：目的:检测不同浓度的消痔灵注射液(Hemorrhoid Injection)对血管瘤内皮细胞(hemangioma endothelial cells)凋亡的影响。方法:设立对照组和不同浓度的消痔灵注射液3个实验组,采用细胞培养MTT法流式细胞术等方法检测不同浓度消痔灵注射液对血管瘤内皮细胞凋亡的作用的影响。结果:对照组及实验组HECS均呈现生长状态,随着药物浓度的增加及时间的延长,细胞形态呈现凋亡改变。消痔灵注射液对血管瘤内皮细胞凋亡的干预与给药剂量及处理时间呈正相关,其能使血管瘤内皮细胞停滞在G0/G1期,从而使血管瘤内皮细胞的死亡率显著增加(P<0.05)。流式分析结果表明给药后血管瘤内皮细胞凋亡率显著增加,差异显著(P<0.05)。结论:消痔灵注射液促进血管瘤内皮细胞凋亡,其凋亡率与药物浓度密切相关。Objective： To detect different concentrations of xiaozhiling injection（ Hemorrhoid Injection） of hemangioma endothelial cells（ hemangioma endothelial cells） apoptosis. Method： To set up the control group and different concentrations of xiaozhiling injection three experimental group,and the methods of cell culture determined by MTT method,flow cytometry to detect different concentrations xiaozhiling influenced by injection of hemangioma endothelial cell apoptosis. Results： The control group and experimental group HECS showed growth state,with the increase of drug concentration and the extension of time,the cell morphology change present apoptosis. Xiaozhiling injection of interference from the hemangioma endothelial cell apoptosis and give medicine dose and treatment time were positively correlated. It can make the stagnation of hemangioma endothelial cells in G0/ G1 phase,so that the mortality of hemangioma endothelial cells increased significantly（ P〈 0. 05）. Flow analysis results showed that the hemangioma endothelial cell apoptosis rate increased significantly after the treatment,with the significant difference（ P〈 0. 05）. Conclusion： Xiaozhiling injection promotes hemangioma endothelial cell apoptosis,the apoptosis rate is closely related to the drug concentration.

关 键 词：血管瘤内皮细胞 消痔灵注射液 细胞凋亡 

分 类 号：R543[医药卫生—心血管疾病]