HPLC-MS法测定去氢骆驼蓬碱在人肝微粒体中代谢产物  

HPLC-MS Analysis of the Metabolites of Harmine in Human Liver Microsome

作　　者：赵爽[1] 王贝贝[1] 田娟[1] 谭鹏[1] 付瑾 刘永刚[1] 

机构地区：[1]北京中医药大学,北京100102 [2]新疆维吾尔医学专科学校,新疆和田830011

出　　处：《中医药学报》2015年第3期11-12,共2页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金(No.30901959)

摘　　要：目的:测定人肝微粒体中去氢骆驼蓬碱的代谢产物。方法:以去氢骆驼蓬碱为研究对象,采用高效液相色谱-离子阱-质谱系统分析去氢骆驼蓬碱经肝微粒体代谢的代谢产物。结果:去氢骆驼蓬碱在人肝微粒体中经Ⅰ相代谢后产物主要为哈尔酚。结论:哈尔酚为首次报道的代谢产物,本研究利用液质联用技术阐明了去氢骆驼蓬碱的体内代谢产物,为进一步研究奠定了基础。Objective：To determine the metabolism of Harmine in human liver microsome.Methods：The metabolites of Harmine in human liver microsome as the research objects were analyzed by HPLC-Trap-MS.Results：The main me-tabolite of Harmine in human liver microsome by phase I metabolites was Harmol.Conclusion：Harmol is firstly reported as the metabolite.The research illustrates the metabolites of Harmine by using LC-MS, and provides the basis for the further study.

关 键 词：肝微粒体 去氢骆驼蓬碱 代谢物 

分 类 号：R284.1[医药卫生—中药学]