温胆汤、化瘀温胆汤对糖耐量低减大鼠PI3K通路作用的研究  

Experimental Research on the Effects of PI3K Pathway in IGT rats by Wendan Decoction and Huayu Wendan Decoction

作　　者：姜广坤[1] 马伯艳[1] 牛雯颖[1] 韩玉生[1] 李雨庭[1] 宋颖星 张广庆[1] 肖洪彬[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2015年第3期36-38,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金青年基金(No.81102545);国家自然科学基金面上项目(No.81473555);黑龙江省自然科学基金(D201263)

摘　　要：目的:利用免疫组化法研究温胆汤、化瘀温胆汤对糖耐量低减(IGT)大鼠脂肪中PI3K、PKB、GSK-3β蛋白表达的影响。方法:40只SD大鼠随机分为空白对照组、IGT模型组、温胆汤组和化瘀温胆汤组,喂养24周后,取脂肪采用免疫组化法测定PI3K、PKB、GSK-3β蛋白含量。结果:与空白对照组比较,IGT组PI3K、PKB蛋白含量显著降低(P<0.05),GSK-3β蛋白含量显著升高(P<0.05)。与IGT组比较,温胆汤、化瘀温胆汤均可显著升高PI3K、PKB蛋白含量(P<0.05),降低GSK-3β蛋白含量(P<0.05)。结论:温胆汤、化瘀温胆汤可以不同程度的作用于IGT大鼠脂肪的PI3K通路。Objective：The experiment aimed to study the protein expressions of phosphatidyl inositol 3-kinase（PI3K）, protein kinase B（ PKB） and glycogen synthase kinase-3β（ GSK-3β） in the tats′fat by immunohistochemistry method. Methods：The SD rats were divided into four groups at random,which were blank control group,IGT model group,Wen-dan decoction group and Huayu Wendan decoction group.Detect the protein expressions of PI3K,PKB and GSK-3βin the fat by immunohistochemical staining.Results：Compared with the blank control group, the protein expressions of PI3K and PKB in fat of IGT group all decreased,but the protein expression of GSK-3βincreased obviously.Conclusion：Wendan decoction and Huayu Wendan decoction can have an effect on different levels of PI3K pathway in the IGT rats.

关 键 词：免疫组化法 IGT PI3K PKB GSK-3β 温胆汤 

分 类 号：R285.5[医药卫生—中药学]