红芪总黄酮对离心运动大鼠骨骼肌的抗氧化作用  

Antioxidative Effect of Total Flavonoids of Hedysarum Polybotry on Skeletal Muscle of Rats after Eccentric Exercise

作　　者：马玉德[1] 杨雅丽[1] 卢翠莲[1] 郭超[1] 彭志辉[1] 王楠[1] 黄彩云[1] 陈彻[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中医药学报》2015年第3期56-58,共3页Acta Chinese Medicine and Pharmacology

基　　金：甘肃省高等学校科研基金项目(No.2014B-057)

摘　　要：目的：研究红芪总黄酮对大鼠离心运动后骨骼肌抗氧化能力的影响。方法：72只Wistar大鼠随机分为安静对照组（ Control group，CG）、离心运动组（ Eccentric Group，EG）和离心运动＋药物预处理组（Drug pretreatment group，DPR）。依据运动后时间点不同再次分为运动前1 h和运动后1，24，48 h 4个亚组，每组6只。离心运动前3天，药物预处理组大鼠每日定时给予0．2mg／kg红芪总黄酮灌胃，其余各组大鼠给予等量生理盐水灌胃。力竭运动后，取各组大鼠腓肠肌，分别检测乳酸脱氢酶（LDH）、活性氧（ROS）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果：离心运动后，大鼠骨骼肌组织中LDH，ROS和MDA含量与运动后明显升高，而SOD活性下降（ P＜0．01～0．05）。红芪总黄酮可延缓离心运动后大鼠骨骼肌ROS、MDA及LDH含量的积累并提高SOD活性，与相应对照组比较，差异具有显著性（ P＜0．05）。结论：红芪总黄酮可有效防止高强度运动后氧化应激所导致的骨骼肌损伤。Objective：To investigate the antioxidative effect of total flavonoids of Hedysarum polybotry on skeletal muscle of rats after eccentric exercise.Methods：Wistar rats were randomly divided into a control group,an eccentric exercise group,a drug pretreatment plus eccentric exercise group.All groups were then randomized into 1h subgroup before exer-cise,1h,24h and 48 h subgroups after exercise respectively,with 6 rats in each subgroup.Rats in the drug pretreatment plus eccentric exercise group were administered by oral total flavonoids of Hedysarum polybotry （ 0 .2 mg/kg ） , once a day.Rats,gastrocnemius muscles in all groups were obtained at different time points.Lactate dehydrogenase （ LDH） , malondialdehyde （ MDA） , reactive oxygen species （ ROS） and superoxide dismutase （ SOD） in the gastrocnemius mus-cles were measured by spectroscopy, respectively.Results： After eccentric exercise, the contents of LDH, MDA and ROS were significantly increased in rats,gastrocnemius muscles （P〈0.05）, and gradually increased over time after ex-ercise.SOD was significantly decreased over time after exercise （P〈0.05）.Total flavonoids of Hedysarum polybotry could decrease the accumulation of LDH, MDA and ROS, and enhance activity of SOD in rats,gastrocnemius muscles. Conclusion：Total flavonoids of Hedysarum polybotry could prevent skeletal muscle injury caused by oxidative stress after high intensity exercise.

关 键 词：红芪总黄酮 离心运动 自由基 抗氧化 

分 类 号：R285.5[医药卫生—中药学]