双波长HPLC法同时测定五加枣仁膏滋中紫丁香苷和斯皮诺素含量测定  

Simultaneous Determination of Syringin and Spinosin Contents in Wujiazaoren Oral Paste by Double Wavelength HPLC

作　　者：许贵军[1] 李迪[1] 吴宏娟[1] 李波 隋金婷[3] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]哈尔滨市食品药品监督管理局,黑龙江哈尔滨150016 [3]哈尔滨市食品药品检验检测中心,黑龙江哈尔滨150025

出　　处：《中医药学报》2015年第3期79-81,共3页Acta Chinese Medicine and Pharmacology

摘　　要：目的：建立一种HPLC法同时测定五加枣仁膏滋中主要有效成分紫丁香苷和斯皮诺素含量。方法：乙腈－0．1％磷酸水溶液－梯度洗脱，检测波长：紫丁香苷检测波长为265 nm、斯皮诺素检测波长为335nm；柱温：40℃。结果：紫丁香苷、斯皮诺素进样量分别在0．16～0．80μg，0．10～0．50μg范围内线性关系良好，相关系数均为0．9999，平均加样回收率（ n＝6）分别为98．81％、99．36％。经测定五加枣仁膏滋中紫丁香苷、斯皮诺素含量分别为0．028％和0．014％。结论：本方法一次性同时测定五加枣仁膏滋中紫丁香苷、斯皮诺素含量，方法简单准确，重复性好，可用于该制剂的质量控制。Objective： To establish a double wavelength HPLC for simultaneous determination of syringin and spinosin contents in Wujiazaoren oral paste.Methods：The analysis was performed on gradient elution by using acetoniterile and 0.1%phosphoric acid, and the flow rate was 1.0mL/min.Syringin and spinosin were detected at 265 nm and 335 nm, respectively.The column temperature was 40℃.Results：The calibration curves of syringin and spinosin showed good-linearity in the ranges of 0.16~0.80μg,0.10~0.50μg.The average recoveries were 98.81%and 99.36%.Conclu-sion：The method is simple, accurate and reproducible for simultaneously determining syringin and spinosin contents in Wujiazaoren oral paste.

关 键 词：五加枣仁膏滋 紫丁香苷 斯皮诺素 

分 类 号：R284.1[医药卫生—中药学]