多菌灵降解菌的鉴定及降解条件初步优化  

Identification of Carbendazim-degrading Bacterium and Degradation Optimization

作　　者：徐惠娟[1] 胡典典 张虹[1] 

机构地区：[1]宁夏大学生命科学学院,银川750021

出　　处：《湖北农业科学》2015年第10期2372-2374,共3页Hubei Agricultural Sciences

基　　金：宁夏自然科学基金项目(NZ13032)

摘　　要：对已分离到的多菌灵降解菌进行16S r DNA序列分析后,采用均匀设计法,优化多菌灵降解菌降解条件。用4因素6水平混合均匀设计表,综合考察培养时间、接种量、p H、温度对多菌灵降解菌降解能力的影响。通过二次多项式逐步回归得出最终降解条件为:培养时间6 d、接种量10%、p H 4.0、温度为45℃时,最优目标函数Y(降解率)为68.65%。进一步进行盆栽试验,检测该菌在实际应用中的降解效率为59.54%。Using the uniform design,optimized degradation conditions of carbendazim-degrading bacterium. Investigate the effect of incubation time, inoculum, pH, temperature on degradation of carbendazim-degrading bacterium.By quadratic polynomial to obtain the final degradation conditions, the bast degradation rate was 68.65% when incubation time was 6 days, inoculum was 10%, pH 4.0 and 45℃.And the degradation rate was 59.54% in practical applications.

关 键 词：多菌灵降解菌 均匀设计 降解条件优化 盆栽试验 

分 类 号：Q939.9[生物学—微生物学]