响应面法对忍冬藤总黄酮提取工艺的优化  

Optimization of Extracting Total Flavonoids from Lonicera japonica Thunb.

作　　者：姜宁[1] 刘晓鹏[1,2,3] 张俊霞[1] 胡绩伟[1] 李力[2] 蒋晓军[2] 吴皓[3] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]南京医药股份有限公司博士后工作站,南京210012 [3]南京中医药大学博士后流动站,南京210023

出　　处：《湖北农业科学》2015年第10期2459-2462,共4页Hubei Agricultural Sciences

基　　金：国家"十二五"科技支撑计划项目(2011BAI04B04);江苏省"企业博士后集聚计划"项目(2011033);2013年湖北省战略性新兴(支柱)产业人才培养(生物工程)项目

摘　　要：试验考察了乙醇体积分数、液固比、提取时间、提取温度4个因素对忍冬(Lonicera japonica Thunb.)藤总黄酮提取的影响,并采用Box-Behnken试验优化了忍冬藤总黄酮提取工艺。结果表明,4个因素能显著影响忍冬藤总黄酮的提取,并优化得出最佳提取工艺为乙醇体积分数91%、液固比42∶1(m L∶g)、80℃提取2.7 h,此时总黄酮提取率为5.22%,与理论值5.24%相差不大,表明该工艺可行。The effects of ethanol concentration,liquid-solid ratio, and extraction temperature, as well as extraction time on ex- traction of total flavonoids from Lonicera japonica Thunb. were investgated. The process of total flavonoids extraction from Lonicera japonica Thunb. was optimized by Box-Behnken experiment. The results showed that four factors including ethanol concentration, liquid-solid ratio, extraction temperature, and extraction time affected the yield of total flavonoids from Lonicera japonica Thunb. significantly. The optimized process was ethanol concentration 91%, liquid-solid ratio 42：1 （mL/g）, extraction temperature 80℃, and extraction time 2.7h. The extraction rate of total flavonoids was 5.22%, compared to the theoretical yield of 5.24%, which was validated well. In conclusion, the optimized technology was feasible.

关 键 词：忍冬(Lonicera JAPONICA Thunb.)藤 总黄酮 提取 工艺优化 

分 类 号：S567.19[农业科学—中草药栽培] R284.2[农业科学—作物学]