山银花多糖提取工艺参数优化  

Optimization of Extraction Parameters of Polysaccharide in Lonicera hypoglauca

作　　者：杨申明[1] 王振吉[1] 谢启明[1] 

机构地区：[1]楚雄师范学院化学与生命科学学院/云南省高校应用生物学重点实验室,云南楚雄675000

出　　处：《湖北农业科学》2015年第10期2466-2469,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31300370);云南省应用基础计划项目(2011FZ186);云南省应用基础计划项目青年项目(2012FD049);楚雄师范学院重点建设学科基金项目(05YJJSXK03);云南省高校科技创新团队支持计划项目(IRTSTYN);楚雄师范学院基金项目(10YJYB02)

摘　　要：采用苯酚-硫酸法测定山银花(Lonicera hypoglauca)多糖的含量,并对提取工艺进行优化。在单因素试验的基础上,采用L9(34)正交试验,以山银花多糖提取率为指标,探讨了提取次数、提取温度、提取时间、料液比对山银花多糖提取率的影响。结果表明,最佳提取工艺参数为提取4次、提取温度80℃、提取时间150 min、料液比1∶20(g∶m L)。在此条件下,山银花中多糖平均提取率为15.39%,加样回收率为97.70%,该方法提取山银花多糖操作简便、准确度高、重现性好,可作为山银花多糖提取和含量测定的方法。In this study, the content of polysaccharide in Lonicera hypoglauca was determined by phenol sulfuric acid method and the parameters of extraction process was optimized. Based on the single factor experiment, using L9（34） orthogonal exper- iment, with the content of polysaccharide in Lonicera hypoglauca as the indicator, effect of extraction times, extraction tem- perature, extraction time, solid-liquid ratio on the extraction rate was studied. The results showed that the optimum extraction process parameters were as followed： extracting 4 times, extraction temperature 80℃, extraction time 120 min, ratio of solid to liquid 1：20 （g/mL）. Under such conditions, the average extraction rat of polysaccharide in Lonicera hypoglauca was 15.39%, the average recovery rate was 97.70%. The method for extraction of polysaccharide in Lonicera hypoglauca had the advantages of simple operation, high accuracy, good reproducibility. Therefore it can be used in the extraction and determi- nation of the content of the polysaccharide.

关 键 词：山银花(Lonicera hypoglauca) 多糖 苯酚-硫酸法 正交试验 

分 类 号：S567.19[农业科学—中草药栽培] R284.2[农业科学—作物学]