UPLC-MS/MS法对香蕉果肉中吡唑醚菌酯残留的测定  

Determination of Residue Pyraclostrobine in Banana by UPLC-MS/MS

作　　者：赵方方[1,2] 谢德芳[1,2] 张月[1,2] 

机构地区：[1]中国热带农业科学院分析测试中心,海口571101 [2]中国热带农业科学院海南省热带果蔬产品质量安全重点实验室,海口571101

出　　处：《湖北农业科学》2015年第10期2477-2479,共3页Hubei Agricultural Sciences

基　　金：农业部农药残留试验项目(2013F285);中国热带农业科学院分析测试中心主任基金项目(2013-01)

摘　　要：建立了吡唑醚菌酯在香蕉全蕉及蕉肉中残留量的超高效液相色谱-串联质谱（UPLC-MS/MS）分析方法。试样经乙腈提取,Strata/NH2固相萃取柱净化,采用UPLC-MS/MS测定。方法最小检出量为4×10-13g,方法定量限为0.2μg/kg;在0.01-0.10 mg/kg范围内,吡唑醚菌酯在香蕉全蕉中平均回收率为83%-92%,相对标准偏差为2.0%-2.8%;在蕉肉中平均回收率为85%-98%,相对标准偏差为1.5%-2.2%。该法灵敏、准确,快速,可用于香蕉果肉中吡唑醚菌酯残留量的测定。The UPLC-MS/MS （Ultra performance liquid chromatography tandem mass spectrometry） method for detecting pyra- clostrobine in banana was established. The sample was extracted from banana with acetonitrile and clean-up using a strata/NH2 SPE column. Then the sample was analyzed by UPLC-MS/MS.The detection limit for pyraclostrobine was 4×10^-13 g, and the limit of quantitation was 0.2 μg/kg. The RSDs in fruit were in the range of 2.0%-2.8%, and recoveries were in the ranges of 83%-92% with 0.01-0.10mg/kg additive amount. Similarly,the RSDs found in flesh were in the range of 1.5%-2.2%, and recovery were in the ranges of 85%-98%. The method is sensitive,accurate,and rapid for detecting pyraclostrobine in banana.

关 键 词：超高效液相色谱-串联质谱(UPLC-MS/MS) 香蕉 吡唑醚菌酯 

分 类 号：TQ450.2[化学工程—农药化工]