基于psbA-trnH序列的剑麻H.11648原始母本的鉴定  

Identification of Original Female Parents of Agave hybrid No.11648 Based on psbA-trnH

作　　者：金刚[1] 陈涛 苏文潘[1] 黄强[1,2] 覃旭[1] 吕平[1] 覃剑峰[1] 王春田[1,2] 

机构地区：[1]广西壮族自治区亚热带作物研究所,南宁530001 [2]广西亚热带优势作物繁育及综合开发新技术重点实验室,南宁530001

出　　处：《湖北农业科学》2015年第10期2513-2515,共3页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(2013GXNSFBA019116);国家现代农业产业技术体系建设项目(CARS-19-S15);广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目(桂热研201206)

摘　　要：剑麻H.11648(Agave hybrid No.11648)是以蓝剑麻(A.amaniensis)和假菠萝麻(A.angustifolia)为亲本进行杂交,F1再与蓝剑麻回交选育而来。分析了剑麻H.11648及其两个亲本叶绿体psb A-trn H序列的差异。结果表明,剑麻H.11648与蓝剑麻的psb A-trn H存在1个明显的SNP差异,而在该位点与假菠萝麻保持一致,从叶绿体DNA水平证实了剑麻H.11648的细胞质来源于假菠萝麻。Agave hybrid No.11648 （H.11648） was derived from the cross between A. amaniensis and A. angustifolia and backcrossed with A. arnaniensis. The difference of psbA-trnH between H.11648 and its patents were analyzed by PCR ampli- fication and sequencing. The sequence analysis showed a C/T SNP loci between the psbA-trnH of A. amaniensis and H. 11648. The results indicated that the cytoplasm of H.11648 derived from A. angustifolia.

关 键 词：剑麻H.11648(Agave hybrid No.11648) 蓝剑麻(A.amaniensis) 假菠萝麻(A.angustifolia) PSBA-TRNH 

分 类 号：S563[农业科学—作物学]