益气养血补肾方对长期大强度运动大鼠海马JAK/STAT信号转导通路的影响  

Effects of Tonifying qi Nourishing Blood and Reinforcing Kidney Recipe on the JAK / STAT signaling pathway in rat hippocampus with long- term high intensity exercise

作　　者：吴春燕[1] 程静静 刘显斌 杜更[3] 

机构地区：[1]山东省体育科学研究中心,山东济南250102 [2]山东省水上运动管理中心,山东日照276800 [3]华中师范大学博士后流动,湖北武汉430079

出　　处：《山东体育学院学报》2015年第3期57-61,共5页Journal of Shandong Sport University

基　　金：山东省优秀中青年科学家科研奖励基金(BS2013SF026);国家体育总局重点研究领域攻关课题(2014B058)

摘　　要：目的:研究复方中药益气养血补肾方对长期大强度运动大鼠海马JAK/STAT信号转导通路的影响,进一步探讨该中药的神经保护作用及机制。方法:30只雄性SD大鼠随机分为对照组、大强度运动组和中药组,每组10只。其中,大强度运动组和中药组进行递增负荷跑台训练,时间为7周。用TUNEL法检测大鼠海马细胞凋亡情况,用Western-blotting法测定海马磷酸化JAK1、STAT1及Casepase-3的蛋白表达水平。结果:与对照组相比,大强度运动组细胞凋亡水平和p-JAK1、p-STAT1和Casepase-3的蛋白表达水平均显著升高(P<0.01);中药组细胞凋亡水平和p-JAK1、p-STAT1和Casepase-3的蛋白表达水平则明显低于大强度运动组(P<0.05,P<0.01)。结论:长期大强度运动可能激活JAK/STAT信号转导通路,发挥促凋亡作用,参与脑缺血损伤后的病理生理过程;复方中药益气养血补肾方可能通过抑制pJAK1、p-STAT1和Casepase-3的表达发挥脑缺血后的抗凋亡作用,从而达到神经保护作用。Objective： To study the effects of compound Chinese medicine on the signal pathway of JAK / STAT in rat hippocampus with long- term high intensity exercise. Methods： Male SD rats were randomly divided into three groups： control group（ n = 10）,high intensity exercise group（ n = 10）and Chinese medicine group（ n = 10）. The exercise project was applied by 7 weeks treadmill at progressive increase exercise intensity. The level of apoptosis in rat hippocampus was detected by the methods of TUNEL. The level of protein expression of p- JAK1,p- STAT1 and Casepase- 3 were determined by the methods of Western blotting. Results： Compared with the control group,the level of apoptosis and protein expression of p- JAK1,p- STAT1 and Casepase- 3 in high intensity exercise group were obviously increased. The level of apoptosis and protein expression of p- JAK1,p-STAT1 and Casepase- 3 in Chinese medicine group were significantly decreased compared with those in high intensity exercise group. Conclusions： The JAK / STAT signal pathway which may be activated by long- term high intensity exercise participates in pathophysiologic process of cerebral ischemia by promoting apoptosis possibly. The compound Chinese medicine can reduce the expression of p- JAK1,p- STAT1 and Casepase- 3 and play an important role in antiapoptosis so as to protect the brain from ischemia injury probably.

关 键 词：长期大强度运动 海马 细胞凋亡 JAK/STAT信号通路 益气养血补肾 

分 类 号：G804.7[文化科学—运动人体科学]