有氧运动对心肌梗死大鼠心脏交感神经重构的影响  

Roles of aerobic exercise on sympathetic neural remodeling in rat with myocardial infarction

作　　者：邵承颖 苏冉[2] 王琳[3] 

机构地区：[1]山东省举重摔跤柔道运动管理中心,山东济南250102 [2]济南市第一人民医院,山东济南250011 [3]山东体育学院研究生处,山东济南250102

出　　处：《山东体育学院学报》2015年第3期73-77,共5页Journal of Shandong Sport University

摘　　要：目的:探讨8周有氧运动对心肌梗死大鼠心脏交感重构的影响及其机制。方法:42只健康雄性SD大鼠随机分为假手术组、心肌梗死组、心肌梗死+有氧运动组。结扎冠状动脉左前降支制备大鼠心肌梗死的模型,术后1周心肌梗死+有氧运动组成活大鼠进行8周跑台运动。采用免疫组织化学方法观察心肌中GAP43、TH阳性神经纤维分布及表达;采用western blot检测心肌中NGF蛋白表达;采用实时定量RT-PCR检测IL-1β及TNF-αmRNA表达。结果:与假手术组大鼠相比,心肌梗死组心肌中GAP43、TH阳性神经纤维密度明显增加(P<0.01),形态粗大且空间分布紊乱;NGF蛋白,IL-1β及TNF-αmRNA表达显著上调。有氧运动干预后心肌组织神经纤维密度明显降低(P<0.01),形态更趋于正常;心肌中NGF、IL-1β及TNF-α表达显著下降。结论:有氧运动减少心肌梗死后心肌局部炎症反应,下调梗死灶周组织NGF表达,抑制交感神经过度再生,从而改善心肌梗死后交感神经重构。Objective： To investigate the role of aerobic exercise in ameliorating sympathetic sprouting after myocardial infarction（ MI） in rat hearts. Methods： 42 male Sprague- Dawley rats were randomly divided into three groups： sham group,MI and MI with aerobic exercise（ MI ＋ ME） group.An MI model was induced by ligation of the coronary artery; the rats in MI ＋ ME group were assigned to 8 weeks of exercise. Immunohistochemistry was used to measure the density of nerve fibers positive for growth- associated protein 43（ GAP43） and tyrosine hydroxylase（ TH）. The protein levels of nerve growth factor（ NGF） were detected by western blot analysis. The mRNA levels of interleukin- 1β（ IL- 1β） and tumor necrosis factor- α（ TNF- α） were examined by quantitative real- time PCR. Results： Compared with the sham- operated hearts,MI increased the density of GAP43- and TH- positive fibers,moreover,the distribution and structure of regenerated nerve was heterogeneous in MI hearts. Moreover,the levels of NGF,IL- 1β and TNF- α were upregulated in MI as compared with sham- operated hearts. Aerobic exercise treatment ameliorated sympathetic hyperinnervation after MI; the levels of NGF,IL- 1β and TNF- α were significantly downregulated with MI ＋ ME than MI alone. Conclusions： Aerobic exercise reduced inflammatory reaction caused by MI,downregulated NGF expression and inhibited sympathetic hyperinnervation,which may be a potential mechanism for ameliorating sympathetic neural remodeling in rat.

关 键 词：有氧运动 心肌梗死 神经重构 炎症 

分 类 号：G804.2[文化科学—运动人体科学]