基于线粒体COI序列对云南地区柚喀木虱单倍型的鉴定  

Identification of haplotypes of Cacopsylla citrisuga from Yunnan Province based on mitochondria COI sequence

作　　者：王妍晶[1] 岑伊静[1] 江宏燕 罗孝竹[1] 邓晓玲[1] XIA Yulu 

机构地区：[1]华南农业大学农学院 [2]Center for Integrated Pest Management,North Carolina State University,1730 Varsity Dr.Suite 110,Raleigh,NC 27606,USA

出　　处：《华南农业大学学报》2015年第4期81-86,共6页Journal of South China Agricultural University

基　　金：美国北卡罗来纳州立大学与华南农业大学合作项目Asian Citrus Psyllid/Citrus Greening Research in China;公益性行业(农业)科研专项(201003067-01)

摘　　要：【目的】研究采自云南的柚喀木虱Cacopsylla citrisuga Yang&Li种群的遗传多样性.【方法】利用线粒体细胞色素C氧化酶亚基I基因(COI)条形码片段对采自云南瑞丽和石屏的柚喀木虱样本进行描述,并进行遗传距离和系统发育分析.【结果和结论】经鉴定共得到2种单倍型C1和C2,瑞丽种群的单倍型均为C1,而石屏的种群分化出了2种单倍型C1和C2.分析表明,柚喀木虱的最大序列分歧度特别低,仅为0.16%.此COI片段能很好地区分柚喀木虱与同属的其他几种木虱.【Objective】To characterize genetic diversity levels of Cacopsylla citrisuga Yang Li collected from two regions of Yunnan Province. 【Method】The samples of C. citrisuga from Ruili city and Shiping county were identified by the gene barcoding of the mitochondrial cytochrome C oxidase subunit I（ COI）.The phylogenetic tree and genetic distance were obtained.【Result and conclusion】Two haplotypes of C.citrisuga,C1 and C2 were identified. C1 was the only haplotype of the populations from Ruili city,while C1 and C2 both existed in the population from Shiping county. Unlike other previous studies of insects,the COI-based genetic diversity of C. citrisuga was extremely low,with the maximum sequence divergence being only 0. 16%. The COI fragment can be used to distinguish C. citrisuga from the other four Cacopsylla species successfully.

关 键 词：云南 柚喀木虱 RAPD-PCR 线粒体COI 单倍型 遗传多样性 

分 类 号：S433.39[农业科学—农业昆虫与害虫防治]