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机构地区:[1]海南大学环境与植物保护学院,海南海口571101 [2]海南省农业科学院农业环境与植物保护研究所/海南省病虫害防控重点实验室,海南海口571100
出 处:《华南农业大学学报》2015年第4期87-90,共4页Journal of South China Agricultural University
基 金:国家自然科学基金(31360432;31160024;31260424);公益性行业(农业)科研专项(201103018)
摘 要:【目的】探究象耳豆根结线虫Meloidogyne enterolobii对番茄品种的最低接种数量,以及接种量对7个番茄品种致病力及生长的影响.【方法】通过温室大棚盆栽番茄,将孵化的象耳豆根结线虫二龄幼虫悬浮液制成10个不同浓度,通过灌根的方法将悬浮液接种至番茄根部,60 d后,测定番茄植株高度、地上部分鲜质量、根结数量、卵块数量和根内幼虫总数量,比较致病力差异.【结果和结论】象耳豆根结线虫二龄幼虫最低接种量为0.08条·cm-3,与其他不同种类的根结线虫相比,具有更强的致病力.象耳豆根结线虫强致病力对番茄抗性和易感病品种均造成严重伤害.【Objective】To explore the minimum inoculation quantity of Meloidogyne enterolobii to tomato and to evaluate the inoculation quantity effects on the growth and pathogenicity of 7 tomato varieties.【Method】The tomato plants were potted in the greenhouse. Ten different gradient suspensions of the second instar larvae were set and inoculated to tomatoes by root-drenching method. The height,ground fresh mass of tomato,the number of root-knots,the number of eggs and the number of juveniles within roots were measured to determine the pathogenicity after sixty days inoculation. 【Result and conclusion】The results showed that the minimum inoculation quantity reached or over 0. 08 of the second instar larvae of M. enterolobii in each cm3 soil,which means strong pathogenicity compared to other species of Meloidogune. The strong pathogenicity of M. enterolobii can cause serious damage to both resistant and susceptible tomato cultivars.
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