川藏黑猪与长白猪仔猪猪瘟母源抗体消长规律比较研究  

DYNATICS OF MATERNAL ANTIBODIES AGAINST CLASSICAL SWINE FEVER IN NATIVE SICHUAN-TIBET BLACK PIGS AND LANDRACE PIGS

作　　者：于吉锋[1] 李江凌[1] 王文贵[1] 廖党金[1] 魏甬[1] 李兴玉[1] 林毅[1] 叶健强[1] 

机构地区：[1]四川省畜牧科学研究院,成都610066

出　　处：《中国动物传染病学报》2015年第3期61-64,共4页Chinese Journal of Animal Infectious Diseases

基　　金：国家现代农业产业技术体系四川创新团队(SCCXTD-004);四川省农业科技成果转化项目(2013NC0016);四川省公益性科研院所基本科研项目(SASA2012A02;SASA2013A04;SASA2013B04)

摘　　要：为探索川藏黑猪和长白猪的猪瘟免疫方法,本研究采用猪瘟阻断ELISA对0、1、4、7、10、20、25、30、35日龄时川藏黑猪与长白猪仔猪的猪瘟母源抗体水平进行了检测。结果显示,川藏黑猪仔猪和长白猪仔猪出生后哺母乳前的猪瘟母源抗体均为0,哺母乳后d1,猪瘟母源抗体分别为>210和28.2,合格率分别为100%和87%。4日龄和7日龄时,川藏黑猪仔猪的猪瘟母源抗体仍为≥210,合格率100%;长白猪仔猪的猪瘟母源抗体为28.4和27.4,合格率100%和83%;随后川藏黑猪仔猪的猪瘟母源抗体呈下降趋势,35日龄达到保护临界值(24.0);长白猪仔猪的猪瘟母源抗体下降更迅速,25日龄时其母源抗体水平已达临界值(24.0)。检测结果表明无论是母源抗体水平还是母源抗体持续时间,川藏黑猪与长白猪仔猪之间有较大差异,川藏黑猪仔猪猪瘟疫苗的首免时间应为35日龄,而长白猪仔猪猪瘟疫苗的首免时间应为25日龄。In order to better understand immunization protocol of classical swine fever in native Sichuan-Tibet black pigs and imported Landrace pigs, maternal antibodies of piglets were monitored at day 0, 1, 4, 7, 10, 20, 25, 30 and 35 by the blocking ELISA. There were no maternal antibodies detected in piglets born to Sichuan-Tibet black sows and Landrace sows before suckling. However, the maternal antibodies were titrated to be 210 or 28.2 in one-day old piglets born to Sichuan-Tibet black sows or Landrace sows, respectively. At day 4 and 7, the maternal antibody titers were 210 in Sichuan-Tibet black piglets while the titers were 28.4 and 27.4 in Landrace piglets. Afterwards, the titers declined to 24.2 in 35 days-old Sichuan-Tibet black piglets and to 24.0 in Landrace piglets. Obviously, the maternal antibodies dropped faster in Landrace piglets than Sichuan-Tibet black piglets. In conclusion, significant differences in titers and duration of maternal antibodies were detected between Sichuan-Tibet black piglets and Landrace piglets. These results suggested that the first immunization could be administered to 35 days-old Sichuan-Tibet black piglets but to 25 days-old Landrace piglets.

关 键 词：猪瘟 母源抗体 消长规律 川藏黑猪 长白猪 

分 类 号：S852.651[农业科学—基础兽医学]