基于大豆回交导入系的脂肪酸组分QTL定位  

Quantitative trait loci mapping of fatty acid based on soybean backcross introgression lines

作　　者：南金平 邹筱[1] 韩粉霞[1] 陈明阳[1] 孙君明[1] 闫淑荣[1] 杨华[1] 

机构地区：[1]中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室/国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《中国油料作物学报》2015年第3期253-261,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家转基因生物新品种培育重大专项(2014ZX08004-004,2013ZX08004-004,2011ZX08004-004);国家科技支撑计划项目(2011BAD35B06);中国农业科学院科技创新工程项目;国家863计划(2012AA101106)

摘　　要：对不同环境条件下大豆脂肪酸主要组分进行QTL定位,为大豆脂肪酸分子标记辅助育种提供理论依据。以中黄13×东山69回交导入系群体BC2F2的100个家系为材料,分析回交群体的SSR标记多态性,结合气相色谱技术测定脂肪酸主要组分含量。构建了一张包含130个SSR标记的大豆遗传连锁图谱,图谱总长2433.29cM,包含19个连锁群,标记间平均遗传距离为18.86cM。共检测到与5种脂肪酸含量相关的QTL47个,其中有21个QTL被重复检测到;QTLqFA-C2-4、qFA-D1b-1、qSA-J-1和qFA-O-1连续3年均被检测到。其中QTLqFA-C2-4检测到与亚麻酸相关,QTLqFA-D1b-1和qSA-J-1均与硬脂酸相关,而QTLqFA-O-1同时检测到与油酸和亚油酸相关。QTLqFA-C2-4、qFA-D1b-1、qSA-J-1和qFA-O-1是本研究中新发现的QTL。此外,QTLqFA-D1a-1和qFA-C2-4是定位到的与多种脂肪酸相关的新QTL。For molecular marker assistant breeding on higher soybean fatty acid,quantitative trait loci（QTL） mapping of predominant fatty acid components in seeds were carried on 100 BC2 lines of backcross introgression lines from Zhonghuang 13 and Dongshan 69. Gas chromatography was used to determine fatty acid components. Re-sults included a linkage map constructed with 130 polymorphism SSR markers. The total distance of the map was 2 433. 29 cM containing 19 linkage groups,and the average distance between markers was 18. 86 cM. Total 47 QTLs related to 5 fatty acid components were detected,and 21 of them were repeatable QTLs. QTL qFA - C2 - 4, qFA - J - 1,qFA - D1b - 1 and qFA - O - 1 appeared consistently over the 3 year period. Among them,qFA - C2- 4 was mapped for linolenic,qSA - J - 1 and qFA - D1b - 1 were for stearic,qFA - O - 1 were for both oleic and linolenic. QTL qFA - D1a - 1 and qFA - C2 - 4 were novel QTLs for several fatty acids. QTL qFA - C2 - 3,qFA- J - 1,qFA - D1b - 1 and qFA - O - 1 were newly reported.

关 键 词：大豆 遗传连锁图谱 脂肪酸 QTL 

分 类 号：S565.103[农业科学—作物学]