菜粕芥子碱酶降解反应的条件优化和机理研究  

Optimization and mechanism of enzymatic degradation of sinapine in rapeseed meal

作　　者：郭勉[1] 郝凯[1] 钮琰星[1] 胡双喜[1] 万楚筠[1] 黄凤洪[1] 

机构地区：[1]中国农业科学院油料作物研究所农业部油料作物生物学与遗传育种重点实验室油料脂质化学与营养湖北省重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2015年第3期360-366,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31071637);国家科技支撑计划(2011BAD26B01-3)

摘　　要：芥子碱是菜粕中的主要抗营养因子之一,为了绿色高效地降解芥子碱,选取了漆酶做催化剂,通过在线-紫外可见光谱实时监测的方法,测定了降解反应的动力学数据。该反应的动力学行为符合酶促反应过程,据此推导出酶解反应的米氏方程。利用薄层层析、高效液相色谱等分离手段,得到了纯化的降解产物,并进行了核磁共振波谱分析。根据动力学数据和产物信息,推测出反应机理可能是自由基过程。Rapeseed meal is one of the most important sources of plant proteins,but the antinutritional factors limit its application in feed industry. Sinapine is the major antinutritional factor. In order to remove sinapine effi-ciently and safety,laccase was chosen as catalyst. By employing real - time UV - vis spectrometry,kinetic data of the enzymatic degradation was obtained. The kinetic behavior was highly consistent with enzymatic reaction and Michaelis - Menten equation was deduced. By employing TLC analysis and HPLC,purified degradation products were obtained and characterized by 1HNMR and 13 CNMR. Based on the results of kinetic data and product informa-tion,suggested possible mechanism could be the free radical pathways.

关 键 词：菜粕 芥子碱 漆酶 动力学 反应机理 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]