鬼针草白粉病的病原菌鉴定  

Pathogen identification of Bidens pilosa powdery mildew

作　　者：陈冠州[1] 沈会芳[2] 林壁润[1,2] 王忠文[1] 张景欣[2] 蒲小明[2] 李培谦[1] 黄宁[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广东省农科院植物保护研究所,广东广州510640

出　　处：《广东农业科学》2015年第11期77-79,F0003,共4页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(S2013010012424);国家自然科学基金(31300118;81373320);广东省国际合作项目(2012B050500014);广州市科技计划项目(12C14071616);国家公益性行业(农业)科研专项(201203021)

摘　　要：采用形态学鉴定、致病性鉴定和分子系统学鉴定方法对中国广东地区首次发现的鬼针草白粉病进行病原鉴定研究.结果表明,该病原菌分生孢子梗直立,圆柱形,没有分支,大小为100～160 μm＆#215;11～13 μm,包含1个足细胞,2～4个短细胞;分生孢子串生,圆柱形,大小为20～50 μm＆#215;10～20 μm（长/宽为1.25～3.0）;病原菌接种叶片病症与自然状态一样,形成薄而边缘不明显的白色斑;对其核糖体DNA内转录间隔区（ITS）序列进行扩增、测序获得528 bp序列（GenBank登录号：KJ920100）,经MECA 3.1软件分析,其与来自二孢白粉菌（Golovinomycescichoracearum）的KF453969序列聚为一枝,与其相似度达到99％.本研究表明,广州地区的鬼针草白粉菌为二孢白粉菌（G.cichoracearum）.In order to make clear the phylogenetic evolution relationship of Bidens pilosa powdery mildew detected in Guangzhou, China, morphological, etiological and molecular systematic identification were tested. Results show that conidiophores were measured with 100 - 160 μm × 11 - 13 μm, and they produced 2 to 4 immature conidia in chains with a crenate outline and 1 to 2 straight cells of the conidiophore at the terminal. Conidia were oval-shaped to round- shaped, hyaline, and measured with 20 - 50 μm × 10 - 20 μm （length/width ratio of 1.25 - 3.0）. The fungus appearing on the inoculated plants had the same morphology with those from original diseased plants. Internal transcribed spacer （ITS） sequences of its ribosomal DNA were amplifild by PCR, 528 bp sequences （GenBank： K J920100） were obtained, and sequence analysis by MEGA3.1 software revealed that sequence of pathogenic bacteria and KF453969 from Golovinomyces cichoracearum gather together with 99% of similarity. The results show that pathogen of B. pilosa powdery mildew in Guangzhou is G. cichoracearum.

关 键 词：鬼针草 白粉病 病原鉴定 二孢白粉菌 

分 类 号：S432.44[农业科学—植物病理学]