参卿止痒洗液对2,4-二硝基氟笨所致慢性湿疹模型的药效学研究  

The Study on the Pharmacological Effects of Shengqing Anti-itch Lotion Against Chronic Eczema Model Caused by DNFB

作　　者：吴波[1] 卢登华[1] 杨芳[1] 陈前明[1] 龙莉[1] 

机构地区：[1]成都市第二人民医院皮肤科临床药学科,四川成都610017

出　　处：《四川医学》2015年第6期816-818,共3页Sichuan Medical Journal

基　　金：四川省中医药管理局资助课题(编号:2010-36)

摘　　要：目的研究参卿止痒洗液对慢性湿疹的作用,为临床合理运用提供可靠参考。方法给小鼠皮肤多次涂抹2,4-二硝基氟苯(DNFB)以复制慢性湿疹模型。同时给予不同浓度的参卿止痒洗液观察药物的作用。结果模型组小鼠在激发后背部皮肤逐渐增厚、粗糙、干燥,有血痂,激发部位有少量毛发生长;动物表现出烦躁不安,回头频率增高,小鼠背部皮肤表皮增厚,皮肤炎性细胞浸润明显增多;而参卿止痒洗液2倍临床浓度、参卿止痒洗液临床浓度(原液)、参卿止痒洗液0.5倍临床浓度等各剂量组较模型组的小鼠皮肤增厚减轻,不干燥,激发部位毛发几乎都生长,小鼠行为也较安静,回头舔舐情况也少;参卿止痒洗液可显著抑制DNFB致小鼠皮肤表皮增厚,抑制炎细胞浸润,与模型组比较差异有统计学意义。结论上述结果提示参卿止痒洗液对慢性湿疹具有一定的防治作用,其详细作用机理有待进一步探讨。Objective To study the therapeutic effect of Shengqing anti-itch lotion against chronic eczema,providing reli-able references to clinical treatment. Methods repeatedly paint 2,4-DNFB（dinitrofluorobenzene）on the skin of mice to replicate chronic eczema model, meanwhile add Shengqing anti-itch lotion of different concentration for the purpose of inspect the drug effect. Results After the model mice was stimulated,its back skin gradually thicken and became rough and dry,carrying with blood scab on its surface. And there was just a small amount of hair remaining growing on it. The mice appeared to be agitated and kept turning round frequently,and the skin of its back thicken,skin inflammatory cell infiltration obviously increased. Compared with the skin of mice of model group,the mice skin of dosage groups carrying Shengqing anti-itch lotion that was twice as much as the dosage for clinical application,that was the same as the dosage for clinical application,and that was half of the dosage for clini-cal application,appeared to thicken less seriously and showed no dryness. And almost all hair in the stimulated parts remained growing. As for the behaviors,the mice were relatively quiet,and would not turn round to lick as frequently as the model mice would. Shengqing anti-itch lotion can obviously inhibit DNFB from thickening the skin of mice and inflammatory cell infiltration. There are some differences between dosage groups and model groups statistically（P＆lt;0. 05）. Conclusion Shengqing anti-itch lo-tion possesses certain preventive and therapeutic effect against chronic eczema;the mechanism of action remains to be further dis-cussed.

关 键 词：参卿止痒洗液 慢性湿疹 小鼠 

分 类 号：R285.5[医药卫生—中药学]