寄主和栖境挥发物对花绒寄甲的嗅觉反应及GC-MS分析  

Olfactory response of Dastarcus helophoroides Sharp to the volatiles emitted from hosts,their habitat,and volatile component analysis by GC-MS

作　　者：高悦[1] 李虹兵[2] 曹霞[3] 解春霞[1] 巨云为[3] 徐福元[1] 

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南京森林警察学院,江苏南京210023 [3]南京林业大学,江苏南京210037

出　　处：《江苏林业科技》2015年第3期12-16,共5页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业三新工程项目"松材线虫病生物防控技术集成与示范""LYSX[2013]20;江苏省林业科学研究院青年基金项目"花绒寄甲对寄主的搜寻行为及影响因子分析"(JAF-2012-6)

摘　　要：在实验室条件下,利用四臂嗅觉仪测定了源于3种寄主以及2种寄主栖境健康和蛀道组织的不同质量浓度提取液对花绒寄甲的嗅觉反应。分别用二氯甲烷和乙醚对3种寄主挥发性化合物抽提浓缩液进行GC-MS分析。结果表明：松褐天牛挥发物提取液质量浓度为25,50 mg/m L时,花绒寄甲在处理区的滞留时间显著高于对照;青杨天牛挥发物提取液质量浓度为25,50 mg/m L时,花绒寄甲在处理区的滞留时间和进入次数都显著高于对照;大麦虫挥发物提取液质量浓度为50 mg/m L时,花绒寄甲在处理区的滞留时间和进入次数都显著高于对照。花绒寄甲对寄主栖境蛀道组织比健康组织的嗅觉反应更显著,花绒寄甲对黑松比对毛白杨寄主栖境的嗅觉反应更显著。3种寄主挥发性化合物抽提液中含有多种活性成分,在10-40 min的保留时间峰值较集中,活性物质的主要成分为C21~C36的直链烷烃以及脂肪酸及其衍生物、萜烯类、醇酮类和苯酚类化合物。The olfactory response of Dastarcus helophoroides Sharp to different volatiles from 3 species hosts and 2 species hosts＇ habitats,both healthy and bored,was measured by using a four-armed olfactometer in the laboratory. By gas chromatography-mass spectrometry（ GC-MS）,we analyzed the volatiles from 3 hosts＇ extraction with dichloromethane and ether.The results indicated that the retention time of D. helophoroides in the treatment area with 25 or 50 mg / m L volatile from Monochamus alternatus was significantly longer than that in CK; Its retention time and entry number in the treatment area with both 25 and 50 mg / m L volatile from Saperda populnea were significantly more than those in CK,but significantly more than those in CK with only 50 mg / m L volatile from Zophobas morio. Its olfactory response to the hosts＇ bored habitat was shown preferably,with more inclination to hosts＇ Pinus thunbergii than Populus tomentosa habitat. GC-MS analysis showed many active components in the volatiles,with main peak values from 10 to 40 min at GC-MS graph,including linear paraffins（ C21- C36）,aliphatic acids and their derivatives,terpenes,alcohols,ketones and phenols.

关 键 词：花绒寄甲 松褐天牛 青杨天牛 大麦虫 嗅觉反应 GC-MS 

分 类 号：S763.301[农业科学—森林保护学]