海藻多糖对衰老小鼠的免疫调节作用  

Immunomodulatory effects of seaweed polysaccharide in aged mice

作　　者：黄震[1] 迟秀文[2] 束振华[1] 孙俊生[1] 

机构地区：[1]深圳市龙岗中心医院,广东深圳518116 [2]广东医学院护理学院,广东湛江523808

出　　处：《国际检验医学杂志》2015年第13期1801-1803,1806,共4页International Journal of Laboratory Medicine

基　　金：国家自然科学基金项目(81102450)

摘　　要：目的探讨海藻多糖(PSS)对D-半乳糖(D-gal)所致衰老小鼠的免疫调节作用。方法腹腔注射D-gal制备衰老小鼠模型,同时对衰老小鼠行PSS灌胃,收集腹腔巨噬细胞,检测巨噬细胞分泌一氧化氮(NO)、一氧化氮合酶(NOS)的水平和诱导型一氧化氮合酶(iNOS)mRNA表达水平;计算脾脏指数,观察PSS对小鼠脾脏显微结构的影响;流式细胞术检测衰老模型小鼠脾细胞周期的变化。结果 PSS能促进衰老小鼠巨噬细胞合成NO、NOS和上调iNOS mRNA表达水平,并使衰老小鼠脾脏指数明显增加,脾脏被膜层明显增生。PSS能增加衰老小鼠脾S期和G2/M期细胞。结论 PSS能提高D-gal所致衰老小鼠的的免疫功能,值得进一步研究和开发。Objective To explore the immunomodulatory effects of seaweed polysaccharide（PSS）in aged mice induced by D-ga-lactose （D-gal）.Methods D-gal was injected intraperitoneally to establish the aged mice model,meanwhile the aged mice was intra-gastricly administrated by PSS.Peritoneal macrophages were collected,and macrophage secretion of nitric oxide （NO）,nitric oxide synthase （NOS）levels and expression levels of inducible nitric oxide synthase （iNOS）mRNA were detected.The spleen indexs of the agd mice were calculated,and effects of PSS on spleen microscopic structure of mouse were observed.The changes of spleen cell cycle in aged model mice were detected by flow cytometry assay.Results PSS could enhance macrophage synthesis of NO and NOS of the aged mice and up-regulate the expression of iNOS mRNA levels.And the spleen index of the aged mice increased obviously, the hyperplasia of spleen capsule was obvious.Moreover,PSS could increase the percentage of S phase and G2/M phase cells of the aged mice spleen.Conclusion PSS could enhance the immune function of aged mice induced by D-gal,which is worthy of further study,which development.

关 键 词：海藻多糖 衰老小鼠 免疫调节 

分 类 号：R285.5[医药卫生—中药学]