乙型肝炎病毒核心抗体临床意义再研究  

Further study on clinical significance of hepatitis B virus core antibody

作　　者：张汉奎[1] 王伟佳[1] 卢建强[1] 杜满兴[1] 罗燕香[1] 阮小倩[1] 黄燕华[1] 冯砚平[1] 

机构地区：[1]中山大学附属中山医院检验医学中心,广东中山528403

出　　处：《国际检验医学杂志》2015年第13期1835-1836,1838,共3页International Journal of Laboratory Medicine

基　　金：广东省中山市科技局医疗卫生科研基金项目(20132A114)

摘　　要：目的分析化学发光微粒子免疫分析法检测乙型肝炎病毒核心抗体（HBcAb）在临床上的应用。方法收集2012年1月至2014年11月乙型肝炎两对半检测HBcAb阳性标本16 830份,根据HBcAb的cut off指数（COI值）将其分为3组：1.0~〈9.0组、9.0~〈11.0组、≥11.0组进行统计分析。对乙型肝炎表面抗原（HBsAg）与乙型肝炎表面抗体（HBsAb）均阴性且HBcAb的COI值大于或等于11.0的标本进行乙型肝炎病毒（HBV）DNA检测。结果 COI≥11.0组标本HBsAg（＋）HBsAb（-）检出率（13.84%）明显高于其他表现形式（P〈0.05）;COI值为9.0~〈11.0组各HBsAg、HBsAb表现形式检出率比较差异无统计学意义（P〉0.05）;COI值为1.0~〈9.0组标本HBsAg（＋）HBsAb（-）检出率（0.5%）明显低于其他2组（P〈0.05）。HBsAg（-）HBsAb（-）且COI≥11.0的标本304份,其中HBV DNA阳性64份,阳性率为21.0%。结论 HBcAb检测中,COI≥11和1.0~〈9.0可分别作为判断HBV现症感染和既往感染的参考指标,临床应用时应联合HBV的其他实验室标志物并结合临床综合分析。Objective To analyse clinical application of hepatitis B virus core antibody（HBcAb）detected by using the chemilu-minescence microparticle immunoassay.Methods A total of 1 6 830 specimen with positive HBcAb detected by using the two pairs of semi-hepatitis test from January 2012 to November 2014 were collected,and divided into three groups according to the cut off in-dex（COI）of detection results of HBcAb,including group 1.0-〈9.0,group 9.0-〈1 1.0 and group COI≥1 1.0,and detection re-sults were statistically analysed.The hepatitis B virus（HBV）DNA test was carried out in specimen with negative hepatitis B surface antigen（HBsAg）and hepatitis B surface antibody （HBsAb）and COI≥1 1.0.Results The detection rate of HBsAg（＋）HBsAb（-） （13.84%）was significantly higher than other expression patterns in group ≥1 1.0（P 〈0.05）.There was no statistically significant differences in positive rate among all expression patterns of HBsAg and HBsAb in the group 9.0-〈1 1.0（P 〉 0.05）.The detec-tion rate of HBsAg（＋）HBsAb（-）of group 9.0-〈1 1.0 was significantly lower than that of the other two groups（P 〈0.05）.A total of 304 specimen were HBsAg（-）HBsAb（-）and COI≥1 1,among them 64 specimen were HBV DNA postive and the posi-tive rate was 21.0%.Conclusion In the detection of HBcAb,COI≥1 1 and 1.0-〈9.0 could be reference indicators for diagnosiing current and past HBV infection respectively,which should be combined with other laboratory indicators of HBV clinical data for comprehensive analysis.

关 键 词：乙型肝炎病毒核心抗体 化学发光 DNA 病毒 

分 类 号：R446.6[医药卫生—诊断学] R373.21[医药卫生—临床医学]