联合应用白介素-4突变体及白介素-5可溶性受体对哮喘小鼠支气管肺泡灌洗液及血清中转化生长因子-β1、活化素-A水平的影响  

Effects of interleukin-4 mutant combined with soluble interleukin-5 receptor α on transforming growth factor-β1 and activin-A levels in bronchial alveolar lavage fluid and serum of asthmatic mice

作　　者：孟祥珍[1] 韩月肖 张方琪[1] 李志奎[1] 

机构地区：[1]第四军医大学西京医院呼吸与危重症医学科,西安710032

出　　处：《中华肺部疾病杂志（电子版）》2015年第3期1-4,共4页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：国家自然科学基金资助项目(81070028)

摘　　要：目的探讨联合应用白介素-4突变体(IL-4MT)及白介素-5可溶性受体(s IL-5Rα)对哮喘小鼠支气管肺泡灌洗液(BALF)及血清转化生长因子-β1(TGF-β1)、活化素-A(Act-A)水平的影响。方法50只雌性BALB/c小鼠被随机分为对照组、哮喘组、IL-4MT治疗组、s IL-5Rα治疗组、联合IL-4MT及s IL-5Rα治疗组(简称联合治疗组);哮喘组和各治疗组分别给予卵蛋白(OVA)致敏和激发。其中,各治疗组(IL-4MT治疗组、s IL-5Rα治疗组、联合治疗组)分别于激发前30 min腹腔注射IL-4MT 100μg、s IL-5Rα100μg及IL-4MT、s IL-5Rα各100μg进行干预,对照组和哮喘组给予生理盐水代替;末次激发后收集小鼠BALF、血清;用ELISA法比较各组(BALF)及血清中TGF-β1、Act-A的水平变化。结果与对照组比较,哮喘组小鼠BALF及血清中的TGF-β1、Act-A水平显著升高(P<0.01);与哮喘组比较,各治疗组BALF及血清中TGF-β1、Act-A水平明显下降(P<0.01);与单独治疗组相比,联合治疗组BALF及血清中的TGF-β1、Act-A水平下降地更明显(P<0.05)。结论联合应用IL-4MT及s IL-5Rα可明显降低哮喘小鼠BALF及血清中TGF-β1、Act-A水平,在减轻哮喘小鼠气道重塑方面可能发挥了重要作用。Objective To observe the effects of interleukin-4 mutant（ IL-4MT） combined with soluble interleukin-5 receptor α（ s IL-5 Rα） on transforming growth factor-β1（ TGF-β1） and activin-A（ Act-A） levels in bronchial alveolar lavage fluid（ BALF） and serum of asthmatic mice. Methods Fifty female BALB / c mice were randomly divided into 5 groups： the normal control group,the asthma group,the IL-4MT treatment group,the s IL-5Rα treatment group,the combination of IL-4MT and s IL-5Rα treatment group. Mice in asthma group and treatment groups were sensitized and challenged by OVA. Mice in treatment groups were respectively injected intraperitoneally with IL-4MT 100 μg,s IL-5Rα 100 μg and the combination of IL-4MT 100 μg and s IL-5Rα 100 μg 30 mins before challenged,while mice in normal group and asthma group received equal volume of saline. Then the levels of TGF-β1 and Act-A in BALF and serum were measured by ELISA. Results Compared with the normal group,the levels of TGF-β1 and Act-A in both BALF and serum of the asthma group were remarkably higher（P〈0. 01）. Compared with the asthma group,treatment groups can effectively reduce the levels of TGF-β1 and Act-A（P〈0. 01）. Compared with single treatment,the combined treatment group were more effective（P〈0. 05）. Conclusion IL-4MT and s IL-5Rα could reduce TGF-β1 and Act-A levels in BALF and serum of asthmatic mice,which could be a useful therapeutic strategy to alleviate the airway remodeling of asthma.

关 键 词：支气管哮喘 白介素-4突变体 白介素-5可溶性受体α 转化生长因子-Β1 活化素-A 

分 类 号：R563[医药卫生—呼吸系统]