红外光谱结合相关性分析对番茄病害叶的研究  

Investigation of Disease Tomato Leaves by Fourier Transform Infrared Spectroscopy Combined with Correlation Analysis

作　　者：杨春艳[1] 刘飞[1] 李开毅[1] 梁云峰 

机构地区：[1]玉溪师范学院物理系,云南玉溪653100 [2]元江县农业经济管理服务中心,云南元江653300

出　　处：《湖北农业科学》2015年第9期2120-2122,2126,共4页Hubei Agricultural Sciences

基　　金：云南省教育厅基金项目(2013Y480);玉溪师范学院青年教师资助计划项目

摘　　要：用傅里叶变换红外光谱结合相关性分析研究番茄正常叶片和3种病害叶片。结果表明,它们的主要物质成分为蛋白质、多糖和脂类物质。正常叶片、叶霉病、晚疫病和煤污病叶片的光谱整体相似,仅在1 750~700 nm,正常叶片与病害叶片光谱的峰位、峰形及吸收强度比有一定差异,吸收强度比A1 102 cm^-1/A1 636 cm^-1为0.727、0.515、0.591和0.719,表明病害叶片中蛋白质的相对含量升高,而糖类物质的相对含量降低。为提高光谱灵敏度,对光谱进行二阶导数相关性分析,4个样品的二阶导数光谱在1 750~1500 cm^-1和1 200~900 cm^-1的相关系数差异明显,表明4种叶片样品所含化学成分结构或各成分相对含量各不相同,即病害影响了番茄叶片的化学成分。上述研究结果表明,傅里叶变换红外光谱结合相关性分析在鉴别番茄病害叶片方面具有方便、快捷等优点。Fourier transform infrared spectroscopy （FTIR） and correlation analysis were used to identify normal and three kinds of disease tomato leaves.The results show the main components of them are protein,polysaccharide and aliphatic compound. spectra of four kinds of leaves are similar,only in the range of 1 750-700 cm^-1 ,the location, the shape and alosorbance ration of absorbance peaks have somewhat varying, the absorbance ratios A1102cm^-1 /A 1636cm^-1 are 0.727,0.515,0.591 and 0.719 in the samples of normal leaves,leaf mold, late blight and sooty blotch leaves are 0.330,0.233,0.327 and 0.302,respectively,which suggest that the diseased leaves have a higher protein content and a lower polysaccharide content than normal leaves.In order to enhance the spectral resolution,the second-derivative spectra were selected for evaluating the correlation,the obvious differ- ence of the correlation coefficients were observed in two ranges of 1 750~1 500 cm^-1 and 1 200~900 cm^-1 for all the four sampies,which indicates that the structure or relative amount of chemical composition have been infected by disease.

关 键 词：傅里叶变换红外光谱 番茄病害 叶片 相关分析 

分 类 号：O657.3[理学—分析化学]