他达拉非延缓大鼠慢性移植血管硬化病变  

Tadalafil attenuates chronic allograft vasculopathy in rat

作　　者：徐自强[1] 夏鹏[1] 王瑾珺[1] 金昊[1] 杨亦荣[1] 

机构地区：[1]温州医科大学附属第一医院器官移植中心,325000

出　　处：《实用医学杂志》2015年第11期1755-1758,共4页The Journal of Practical Medicine

基　　金：浙江省教育厅基金资助项目(编号:Y201326684);温州市科技计划资助项目(编号:Y20120157);浙江省科技厅实验动物平台项目(编号:2013C37006)

摘　　要：目的:探讨他达拉非对大鼠慢性移植动脉血管病变保护作用。方法:实验分3组:同系移植组,供、受者为Lewis大鼠,无处理;异系移植实验组,Lewis大鼠接受Brown-Norway(BN)大鼠腹主动脉移植;干预组他达拉非0.5 mg/(kg·d),Lewis大鼠接受BN大鼠腹主动脉移植。术后4周和8周取移植动脉,组织学观察、测量内膜和中膜厚度,Western blot检测PKG-Ⅰ、Rho A蛋白表达。收集各组血浆,ELISA检测c GMP浓度。结果:异系移植实验组移植动脉呈移植物血管病表现,血管内膜显著增厚;干预组血管内膜增厚,与实验组相比显著减轻(P<0.05);干预组血浆c GMP浓度较实验组显著增高(P<0.05);与实验组相比,干预组PKG-Ⅰ显著增高、Rho A明显降低(P<0.05)。结论他达拉非通过调控c GMP/PKG-Ⅰ和Rho A通路,抑制移植物血管病发展。Objective To evaluate the effects of tadalafil on chronic allograft vasculopathy. Method The abdominal aorta transplantation was performed on Male Lewis or Brown-Norwai rats as donors and male Male Lewis rats as recipients. The recipients were divided into 3 groups： Group A （Lewis-Lewis）with no treatment ; Group B （BN-Lewis） with no treatment ; Group C （BN-Lewis） received tadalafil treatment （0.5 mg/kg per day）. The rats were sacrificed at 8 weeks post treatment. The grafted aortas were used for histology and Western blot assay. Plasma cGMP level was detected by ELISA assay. Results The aortas intimal in group C was significantly thinner than that in group B. PKG- I protein expression in group C was significantly higher than that in group B. Expression of RhoA in group C was lower than that in group B. Conclusion Tadalafil has positive effect on chronic allograft vasculopathy.

关 键 词：他达拉非 移植物闭塞 移植 

分 类 号：R965[医药卫生—药理学]