免疫荧光层析法测定糖化血红蛋白的效果评价  

Evaluation on Immune Fluorescence Chromatography for Glycosylated Hemoglobin

作　　者：曹东林[1] 雷达[1] 张知洪[1] 王婷[1] 孙亮[1] 

机构地区：[1]广东省第二人民医院检验科,广州市510317

出　　处：《实用医学杂志》2015年第11期1849-1851,共3页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金项目(编号:C2012036);2013年度广东省级财政技术研究开发与推广应用专项资金项目(编号:粤财工[2013]401号)

摘　　要：目的:评价免疫荧光层析法定量检测糖化血红蛋白的效果。方法:用6.0%和8.0%定值样本评价免疫荧光层析法的精密度。以高效液相色谱法作为对照组,免疫荧光层析法检测200份EDTA-K2抗凝全血Hb A1c,进行同步盲法比对试验。结果 :荧光免疫层析法的精密度在6.0%和8.0%定值样本的变异系数分别为5.1%和5.3%。免疫层析荧光法与HPLC法的线性回归方程为y=-0.110+1.021x,相关系数r=0.982;以6.0%和8.0%作为cut-off值,得kappa值分别为0.950(P<0.001)和0.922(P<0.001)。结论 :免疫荧光层析法与HPLC法检测Hb A1c具有一致性,可用于Hb A1c的临床检测。Objective To evaluate the effect of immune fluorescence chromatography on glycosylated hemoglobin （HbAlc）. Methods The precision of immune fluorescence chromatography was evaluated with samples of 6.0% and 8.0% fixed value. Group of High performance liquid chromatography （HPLC） as control, HbAlc for 200 samples of EDTA-K2 anti-coagulated whole blood were detected by immune fluorescence chromatography to synchronous blinded trial. Results As to the precise of immune fluorescence chromatography in the samples of 6% and 8%, values of coefficient of variation were 5.1% and 5.3%, respectively. The linear regression equation of immune fluorescence chromatography and HPLC was Y = -0.110 ＋ 1.021X and the correlation coefficient was 0.982. 6.0% and 8.0% as the cut-off value, kappa values were 0.950 （P 〈 0.001） and 0.922 （P 〈 0.001 ）, respectively. Conclusion Immune fluorescence chromatography and HPLC is consistent with detection of HbAlc, which can be used for clinical detection of HbAlc.

关 键 词：糖化血红蛋白 免疫荧光层析 高效液相色谱 

分 类 号：R446.6[医药卫生—诊断学]