款冬酮抗过敏作用研究  

Antiallergic effect of tussilagone from Tussilago farfara L.

作　　者：李艳芳[1] 陈雪园[1] 熊卫艳 张如松[1] 杨苏蓓[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江省中药研究所,杭州310023

出　　处：《中国新药杂志》2015年第13期1517-1522,共6页Chinese Journal of New Drugs

基　　金：浙江省自然科学基金(LY15H280002)

摘　　要：目的:评价款冬酮的抗过敏作用。方法:通过CO2超临界流体萃取、硅胶柱层析等方法,从款冬花中分离制备款冬酮;采用透明质酸酶抑制实验、肥大细胞(mast cell,MC)脱颗粒实验、豚鼠离体回肠实验、小鼠被动皮肤过敏反应(PCA)实验及小鼠迟发型超敏反应(DTH)实验,观察款冬酮抗过敏的药理作用。结果:款冬酮能显著抑制透明质酸酶的活性,其高、中、低剂量组的抑制率分别为68.6%,45.3%,29.6%;款冬酮能显著抑制肥大细胞脱颗粒作用,其高、中、低剂量组的抑制率分别为100.0%,51.0%,0%;款冬酮能显著抑制组胺致豚鼠离体回肠收缩作用,其高、低剂量组抑制率分别为64.9%,44.1%;款冬酮高、低剂量对组胺致小鼠皮肤速发过敏反应的抑制率分别52.3%,41.6%;款冬酮高、低剂量对2,4-二硝基氯苯(DNCB)致小鼠耳肿胀抑制率分别为25.6%,6.2%。结论:款冬酮具有较强的抗过敏作用。Objective： To determine the antiallergic effect of tussilagone from Tussilago farfara L. Methods： Tussilagone was separated from Tussilago farfara L. by CO2 supercritical fluid extraction and silica gel column chromatography. Antiallergic effect of tussilagone was evaluated by hyaluronidase inhibition test,mast cell degranulation test,experiment in isolated ileum from guinea pig,experimental passive cutaneous anaphylaxis in mice and delayed-type hypersensitivity in mice. Results： Tussilagone significantly inhibited the activity of hyaluronidase by29. 6%,45. 3% and 68. 6% at 0. 26,0. 77 and 2. 30 mmol·L-1,and mast cell degranulation by 0%,51. 0%and 100. 0% at 1. 92 × 10-5,3. 84 × 10-5and 7. 68 × 10-5mol·L-1,respectively. It inhibited histamine-induced contraction of guinea pig ileum in vitro by 44. 1% and 64. 9% at 1. 54 and 3. 07 mmol·L-1. Tussilagone（ 10. 7and 21. 5 mmol·L-1） inhibited histamine-induced immediate allergic reactions in mouse skin by 52. 3% and41. 6%,and DNCB-induced ear edema by 6. 2% and 25. 6% in mice. Conclusion： Tussilagone has a strong antiallergic effect.

关 键 词：款冬花 款冬酮 透明质酸 肥大细胞 离体回肠 被动皮肤过敏反应 迟发型超敏反应 

分 类 号：R285[医药卫生—中药学]