聚乙烯亚胺修饰多壁碳纳米管及对PC12细胞生物相容性的评价  

Evaluation of modified multi-walled carbon nanotubes with polyethyleneimine and its biocompatibility on PC12 cells

作　　者：张涛[1] 李桂华 李晓娟[1] 韩博[1] 赵文彬[1] 

机构地区：[1]石河子大学药学院,石河子832000 [2]新疆玛纳斯佳园医院,玛纳斯832200

出　　处：《中国新药杂志》2015年第13期1546-1553,共8页Chinese Journal of New Drugs

基　　金：国家自然科学基金(81160395)

摘　　要：目的:聚乙烯亚胺(polyethyleneimine,PEI)功能化修饰多壁碳纳米管(MWCNTs-COOH)制备低毒、水溶性良好的碳纳米管聚乙烯亚胺复合物(MWCNTs-PEI),为碳纳米管作为药物载体研究奠定基础。方法:通过聚乙烯亚胺(PEI)共价修饰羧基化多壁碳纳米管(MWCNTs-COOH)制备得到MWCNTs-PEI,利用扫描电镜(SEM)、透射电镜(TEM)、红外光谱(FT-IR)、热失重分析(TG)、拉曼光谱(Raman)、Zeta电位等测定方法对复合物进行表征;采用四基偶氮噻唑蓝法(MTT)及流式细胞仪法考察其对大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)的毒性作用,评价复合物体外生物相容性。结果:通过SEM,TEM,FTIR,TG,Raman,Zeta电位等测定结果证实PEI成功接枝到MWCNTs上;分散性实验结果表明MWCNT-PEI在去离子水中分散均匀且稳定性良好;MTT及流式细胞实验结果表明通过PEI功能化修饰MWCNTs使得MWCNTs的细胞毒性显著降低。结论:通过PEI功能化修饰后的MWCNTs分散性得到明显改善且对细胞毒性显著降低,具有更好的生物相容性。Objective： To prepare multi-walled carbon nanotubes-polyethyleneimine composite（ MWCNTsPEI）,which has low toxicity and better dispersion,using polyethyleneimine functionally modified multi-walled carbon nanotubes in order to lay a foundation for the further research of drug-loading carriers. Methods： MWCNTsPEI composite was prepared by modifying carboxylated multi-walled carbon nanotubes（ MWCNTs-COOH） with polyeth-ylene（ PEI）. Then,the composite was characterized by scanning electron microscopy（ SEM）,transmission electron microscopy（ TEM）,fourier transform infrared spectroscopy（ FT-IR）,thermal gravimetric analysis（ TG）,Raman spectrumzeta and Zeta potential. The toxicity of the composite on PC12 cells was determined by MTT assay and flow cytometry to evaluate its biocompatibility. Results： The results of SEM,TEM,FTIR,TG,Raman,Zeta and other measuring methods confirmed that the PEI was successfully grafted with MWCNTs. The dispersibility test showed that MWCNTS-PEI had better dispersion stability in water. MTT assay and flow cytometry showed that polyethyleneimine functionally modified multi-walled carbon nanotubes greatly reduced the cytotoxicity of MWCNTs. Conclusion： After multi-walled carbon nanotubes are functionally modified with polyethyleneimine,the dispersibility of the composite will be improved and the cytotoxicity significantly reduced showing a better biocompatibility.

关 键 词：聚乙烯亚胺 多壁碳纳米管 生物相容性 

分 类 号：R94[医药卫生—药剂学]