大鼠脊髓不同平面双侧半横切损伤模型的建立及评价  

Establishment and Evaluation of a Spinal Cord Injury Model with Bilateral Hemi-sections at Different Planes in Rats

作　　者：陈斌[1] 武庚[1] 黄喆[1] 王一更 韩旭[1] 罗元烨 郭亮[1] 赵富生[1] 

机构地区：[1]牡丹江医学院,牡丹江157011

出　　处：《实验动物科学》2015年第1期7-11,16,共6页Laboratory Animal Science

基　　金：黑龙江教育厅课题(12511584);牡丹江医学院学生科研项目(201408)

摘　　要：目的建立一种新型大鼠脊髓切割损伤模型,为临床脊髓不同平面损伤研究提供适宜的动物模型。方法45只SD大鼠随机分为脊髓不同平面双侧半横切组（A组,n=15）、脊髓全横切组（B组,n=15）和假手术组（C组,n=15）。术后观察、记录各组大鼠活动、进食水、排尿排便、切口感染等并发症和死亡情况,采用BBB运动功能评分和斜板试验评价大鼠行为学功能。结果术后A组大鼠活动、进食水量减少,53.33%大鼠出现尿潴留,40.00%大鼠于术后1～4周死亡;B组大鼠活动、进食水量明显减少,66.67%大鼠出现尿潴留,60.00%大鼠于术后1～4周死亡;C组大鼠活动、进食水量无明显变化,无并发症及死亡发生。BBB运动功能评分及斜板维持最大角度值检测显示,A、B组均低于C组（P〈0.05）,而这些评价指标在A组和B组之间无显著差异。结论脊髓不同平面双侧半横切损伤模型大鼠尿潴留及死亡率低,行为学评价与脊髓全横切损伤模型类似,适于脊髓不同平面双侧损伤修复的研究。Objective To establish a novel rat spinal cord injury model so as to furnish a suitable animal model for clinical research of bilateral spinal cord injury at different planes. Method Forty-five Sprague-Dawley rats were randomly divided into bilateral spinal cord hemi-section group（ group A,n = 15）,spinal cord transection group（group B,n = 15） and sham-operation group（ group C,n = 15）. After operation,the activities,ingesta,excretion,wound infection,death and other complications of rats in different groups were observed. Besides,BBB locomotive function scores and inclined plane test were performed to evaluate rats＇ behavioral function. Result After operation,the activities and ingesta were decreased in group A. Meanwhile,53. 33% of rats suffered from the uroschesis and 40. 00% died in the course of 28-days＇ evaluation. In group B,The activities and injesta were obviously decreased,66. 67% of rats suffered from the uroschesis and 60. 00% died in 4 weeks post-injury. In group C,whereas,rats＇ activities and ingsta did not decrease as well as no complications and death occurred. The mean scores of BBB and maximum angle of inclined plane in group A and B were significantly lower than that in group C（ P〈0. 05）,but these parameters had no remarkable difference between group A and group B. Conclusion The spinal cord injury model with bilateral hemi-sections at different levels possesses the distinctions of lower incidence of uroschesis and mortality,and has not significant difference compared with the traditional spinal cord trans-ection model in behavioral evaluation. Thus,it is a suitable model for clinical research on bilateral spinal cord injury at different levels.

关 键 词：大鼠 脊髓损伤模型 双侧半横切 

分 类 号：Q95-33[生物学—动物学]