“调肝肾,祛痰瘀”复方血压峰值前给药对自发性高血压大鼠早期肾损害的影响  

Effect of Tiaoganshen Qutanyu-formula on Kidney Tissue Injury on Early Stage of Spontaneous Hypertensive Rats

作　　者：吴椋冰[1,2] 潘毅[1] 王志国[1] 阳涛[3] 钟婷[1] 李建平[1] 

机构地区：[1]广州中医药大学,广州510405 [2]广西中医药大学,南宁530001 [3]萍乡市人民医院,江西萍乡337000

出　　处：《中国实验方剂学杂志》2015年第14期77-81,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173181)

摘　　要：目的:探讨"调肝肾,祛痰瘀"复方血压峰值前给药对自发性高血压大鼠(SHR)血压和早期肾损害指标的影响。方法:将30只SHR(12周龄),随机分为复方组(4 g·kg-1),氯沙坦组(30 mg·kg-1)和模型组,设SD大鼠10只为正常组,治疗12周后,观察各组大鼠血压,釆用ELISA方法测定尿微量白蛋白(m MALb),尿β2微球蛋白(β2-MG)。全自动生物化学分析仪检测各组大鼠血清肌酐(SCr)和尿素氮(BUN)含量,免疫组化法检测肾组织胶原蛋白I型(Collagen I)和胶原蛋白IV型(Collagen IV)。结果:与正常组比较,模型组血压明显升高,m MALb,β2-MG,BUN和SCr含量明显升高(P<0.01),肾脏中Collagen I和Collagen IV的表达明显升高(P<0.01);与模型组比较,中药复方组有平稳降压作用(P<0.01),能明显降低m MALb,β2-MG和SCr含量(P<0.05,P<0.01),复方组与模型组,氯沙坦组的BUN含量无统计学意义,能明显降低肾脏中Collagen I和Collagen IV的表达(P<0.01),与氯沙坦组比较没有统计学差异。结论:"调肝肾、祛痰瘀"复方血压峰值前给药能够降低SHR血压,改善其肾损害。其作用机制应是在中医阴阳升降机制协同下,通过降低尿MALb,尿β2-MG,血清SCr以及肾脏Collagen I和Collagen IV的表达所致。Objective： To observe the effect of Tiaoganshen Qutanyu-formula （TQF） on blood pressure and kidney tissue injury index on the early stage of spontaneous hypertensive rats （SHR） administered before blood pressure peak. Method： A total of 30 12-week male SHR were randomly divided into 3 groups： the TQF group （4 g ·kg-1 ） ,the losartan group （30 mg ·kg-1 ） and the model group. Another healthy 10 SD rats were taken as the normal group. The blood pressure was observed. Microalbuminuria （mALb） and fl2-urinary microglobulin （β2- MG） were detected by ELISA after 12 weeks of treatment. The serum contents of creatinine （SCr） and urea nitrogen （BUN） were observed. Moreover, collagen I and collagen IV in kidney tissues were detected by using immunohistochemical method. Result： Compared with the normal group, the blood pressure elevated, the levels of mAlb, β2-MG, BUN and SCr increased, the collagen I and collagen IV in kidney tissues increased in the model group （P 〈 0.01 ）. Compared with the model group, the blood pressure declined, the levels of mMALb, β2-MG, BUN and SCr decreased, the collagen I and collagen IV in kidney tissues decreased in the TQF group （P 〈 0.05, P 〈0.01）. There was no statistical difference between the TQF group and the losartan group. Conclusion： TQF taken before blood pressure peak could reduce blood pressure and decrease kidney tissue injury in SHR. The mechanism might be the synergistic effect of Yin-yang theory of inhibiting the expressions of mALb, β2-MG, SCr and collagen I, collagen IV in the kidney.

关 键 词：“调肝肾 祛痰瘀”复方 血压峰值 自发性高血压大鼠 肾损害 

分 类 号：R285.5[医药卫生—中药学]