“调肝肾,祛痰瘀”复方血压峰值前给药对自发性高血压大鼠心肾组织形态学的影响  

Effect of Tiaoganshen Qutanyu-formula on Histomorphology of Heart and Kidney Tissue on Early Stage of Spontaneous Hypertensive Rats

作　　者：吴椋冰[1,2] 潘毅[2] 王志国[2] 阳涛[3] 钟婷[2] 

机构地区：[1]广西中医药大学,南宁530001 [2]广州中医药大学,广州510405 [3]萍乡市人民医院,江西萍乡337000

出　　处：《中国实验方剂学杂志》2015年第14期82-86,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173181)

摘　　要：目的:探讨"调肝肾,祛痰瘀"复方血压峰值前给药对自发性高血压大鼠(SHR)早期心肾损害组织形态学的影响。方法:将30只SHR(12周龄),随机分为复方组(4 g·kg-1),氯沙坦组(30 mg·kg-1)和模型组,另设SD大鼠10只为正常组,治疗12周后观察肾脏和心脏的超微结构和血管紧张素Ⅱ(AngⅡ)蛋白表达。结果:与正常组比较,模型组肾小球系膜细胞核大,胞浆少,周围基膜明显增厚,胶原纤维增生明显,心肌细胞核,核形不规则,在核旁有些空染的区域,肌原纤维溶解消失,周围的线粒体发生了明显的空泡变性,大鼠肾组织和左心室组织AngⅡ的蛋白表达显著升高(P<0.01);与模型组比较,复方组和氯沙坦组均能明显改善SHR的心脏和肾脏超微结构,显著降低AngⅡ在肾脏和心脏的表达(P<0.01)。结论:调肝肾,祛痰瘀"复方血压峰值前给药能够减轻高血压心肾损害病理损伤,其作用可能是在中医阴阳升降机制协同下,通过下调AngⅡ表达,减少细胞外基质(ECM)在心肾组织局部的聚集而实现。Objective： To observe the effect of Tiaoganshen Qutanyu-formula （TQF） on histomorphology of kidney tissue injury index on the early stage of spontaneous hypertensive rats （SHR） administered beibre blood pressure peak. Method ： A total of 30 12-week male SHR were randomly divided into 3 groups： the TQF group （4 g·kg -1 ）, the losartan group （30 mg ·kg-1 ） and the model group. Another healthy 10 SD rats were taken as the normal group. The kidney and heart uhrastructure and protein expression of angiotensin Ⅱ were observed after 12 weeks of treatment. Result： Compared with the normal group, the kidney and heart uhrastructure had varying degrees of injuries, the protein expression of angiotensin II increased in the model group （P 〈 0.01 ）. Compared with the model group, the kidney and heart ultrastructure had good improvement, the protein expression of angiotensin II decreased in the TQF group （P 〈 0.01 ）. Conclusion： TQF taken before blood pressure peak could reduce the pathological injury of kidney and heart. The mechanism might be the synergistic effect of Yin-yang theory of preventing the aggregation of extra cellular matrix by reducing the expression of angiotensin II.

关 键 词：“调肝肾 祛痰瘀”复方 血压峰值 自发性高血压大鼠 组织形态学 血管紧张素Ⅱ 

分 类 号：R285.5[医药卫生—中药学]