“调肝肾,祛痰瘀”复方血压峰值前给药对自发性高血压大鼠肾脏TGF-β_1,CTGF和FN的影响  

Effect of Tiaoganshen Qutanyu-formula on Transforming Growth Factor-β_1,Connective Tissue Growth Factor and Fibronectin in Spontaneous Hypertensive Rats

作　　者：吴椋冰[1,2] 潘毅[1] 王志国[1] 阳涛[3] 冯家伟[1] 邱建华[1] 李建平[1] 邓文思[1] 

机构地区：[1]广州中医药大学,广州510405 [2]广西中医药大学,南宁530001 [3]萍乡市人民医院,江西萍乡337000

出　　处：《中国实验方剂学杂志》2015年第14期87-91,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173181)

摘　　要：目的:探讨"调肝肾,祛痰瘀"复方血压峰值前给药对自发性高血压大鼠肾脏转化生长因子-β1(TGF-β1),结缔组织生长因子(CTGF)和糖蛋白纤维连接蛋白(FN)的影响。方法:将30只SHR(12周龄),随机分为复方组(4 g·kg-1),氯沙坦组(30 mg·kg-1)和模型组,设SD大鼠10只为正常组,治疗12周后,采用RT-PCR和Western blot法分别检测肾组织中TGF-β1,CTGF和FN的m RNA和蛋白表达。结果:与正常组比较,模型组TGF-β1,CTGF和FN m RNA表达均明显上调(P<0.01),CTGF,FN蛋白表达均明显上调(P<0.01);与模型组比较,复方组TGF-β1,CTGF,FN的m RNA的表达均明显下调(P<0.01,P<0.05),TGF-β1,CTGF,FN蛋白表达均明显下调(P<0.01);氯沙坦组TGF-β1,FN m RNA表达均明显下调(P<0.01),TGF-β1和FN蛋白表达均明显下调(P<0.01,P<0.05)。与氯沙坦组比较,复方组TGF-β1的m RNA表达较明显下调(P<0.05),CTGF和FN的蛋白表达亦下调(P<0.05)。结论:"调肝肾,祛痰瘀"复方血压峰值前给药能够明显减少ECM聚积而改善SHR肾损害。其作用机制应是在中医阴阳升降机制协同下,通过降低肾组织TGF-β1,CTGF,FN的表达而达致。Objective： To observe the effect of Tiaoganshen Qutanyu-formula （TQF） on transforming growth factor-β1 （TGF-β1）, connective tissue growth factor （CTGF） and fibronectin （FN） in kidney tissue in spontaneous hypertensive rats （SHR） administered before blood pressure peak. Method： A total of 30 12-week male SHR were randomly divided into 3 groups： the TQF group （4 g·kg-1）, the losartan group （30 mg ·kg l） and the model group. Another healthy 10 SD rats were taken as the normal group. The mRNA and protein expressions of TGF-β1, CTGF and FN were detected by using RT-PCR and Western Blot after 12 weeks of treatment. Result： Compared with the normal group, the mRNA expressions of TGF-β1, CTGF and FN were obviously up-regulated, protein expressions of CTGF and FN were also up-regulated in the model group （P 〈 0.01）. Compared with the model group, mRNA and protein expressions of TGF-β1, CTGF and FN were down- regulated in TQF and losartan groups （P 〈 0.05, P 〈 0.01 ）. Moreover, the results were lower in the TQF group （P 〈 0.05 ）. Conclusion： TQF taken before blood pressure peak could reduce the aggregation of extra celluar matrix and prevent kidney tissue injury in SHR. The mechanism might be the synergistic effect of Yin-yang theory of the expression inhibition of TGF-βl , CTGF and FN in kidney tissue.

关 键 词：“调肝肾 祛痰瘀”复方 血压峰值 自发性高血压大鼠 转化生长因子-β1 结缔组织生长因子 纤维连接蛋白 

分 类 号：R285.5[医药卫生—中药学]