齐墩果酸对H22荷瘤小鼠肿瘤组织中细胞凋亡相关蛋白表达的影响  

Impact of Oleanolic Acid on Apoptosis-related Protein Expression in Mice Bearing H22 Tumor Tissue

作　　者：董静[1] 吴勃岩[2] 刘艳秋[2] 徐放[2] 车艳新 王晓军[2] 

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161006 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《中国实验方剂学杂志》2015年第14期129-131,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省教育厅科研项目(12531610);黑龙江省自然科学基金(D200844)

摘　　要：目的:通过研究齐墩果酸在H22荷瘤小鼠肿瘤组织中细胞凋亡相关蛋白的表达的影响,探讨其在肿瘤发生、发展过程中的可能作用及意义。方法:建立H22荷瘤小鼠实体瘤模型进行体内试验,将50只已接种H22细胞株的小鼠随机分为模型组,环磷酰胺40 mg·kg-1组,齐墩果酸低、中、高剂量组(1.25,2.50,5.00 mg·kg-1),连续灌胃给药10 d;处死小鼠,检测肝/体比及脾脏指数;采用免疫组化法检测瘤体内凋亡抑制基因生存素(survivin)及B细胞淋巴瘤/白血病-2(Bcl-2)的蛋白表达水平;利用原位末端标记法检测凋亡指数。结果:齐墩果酸可改善H22荷瘤小鼠的肝/体比和脾指数,齐墩果酸低、中、高剂量组对H22荷瘤小鼠肿瘤组织中survivin蛋白表达水平比模型组均显著降低(P<0.01);齐墩果酸高、中剂量组对H22荷瘤小鼠肿瘤组织中Bcl-2蛋白表达水平与模型组比较显著降低(P<0.01,P<0.05);细胞经齐墩果酸处理后,survivin,Bcl-2表达下调,齐墩果酸各组细胞凋亡指数与模型组比较均显著升高,差异显著(P<0.01)。结论:齐墩果酸可通过抑制H22荷瘤小鼠肿瘤细胞中survivin及Bcl-2蛋白的表达,提高凋亡率,诱导肿瘤细胞凋亡,还可改善荷瘤小鼠机体免疫力。survivin,Bcl-2异常表达与肿瘤的发生密切相关,通过共同调节凋亡而发挥抗肿瘤作用。Objective： To study effect of oleanolic acid on apoptosis related proteins expression in H22 mice tumor. Method： Mice H22 tumor-burdened tumor model was used in vivo 50 mice inoculated H22 cell lines were randomly divided into model group, eyelophosphamide group, oleanolic acid low, medium and high dose groups （ 1.25, 2.50, 5.00 mg ·kg-1 ）. The detection of liver, body weight ratio and spleen index was performed. Immunohistoehemical method was used to detect the expression of survivin and B cell lymphoma/leukemia-2 （Bcl- 2） protein, in situ end labeling method was used to detect apoptosis index, and analyzes its relevance. Result： Oleanolic acid could improve H22 tumor-burdened mice liver and body ratio and spleen index, the survivin protein expression was significantly lower than that in the model group （P 〈 0.01 ）; oleanolic acid high dose group decreased significantly Bcl-2 protein expression compared with model group （ P 〈 0.01 ） ; apoptosis index was significantly increased compared with model group （P 〈 0.01 ）. Conclusion ： Oleanolic acid can inhibit H22 mice tumor-burdened tumor cells, increase the rate of apoptosis, thus promotes apoptosis. Survivin and Bcl-2 abnormal expression is closely related to the occurrence of tumor, play a role of anti-tumor by regulating apoptosis together.

关 键 词：齐墩果酸 细胞凋亡 生存素 B细胞淋巴瘤/白血病-2蛋白 凋亡指数 

分 类 号：R285.5[医药卫生—中药学]