铁皮枫斗颗粒中人参皂甙Rg1、人参皂甙Re、人参皂甙Rb1含量测定  

Determination of Ginsenoside Rg 1, Ginsenoside Re, Ginsenoside Rb 1 in Tie PiFengDou Granules by HPLC

作　　者：沈春香 杨兵勋 杨芳亮 

机构地区：[1]浙江天皇药业有限公司,浙江杭州310012

出　　处：《中国科技期刊数据库 医药》2015年第5期44-44,46,共2页

摘　　要：建立同时测定铁皮枫斗颗粒中人参皂甙Rg1、人参皂甙Re、人参皂甙Rb1含量的方法。方法:样品用水饱和正丁醇溶液提取,采用UltimateAQ-C18色谱柱(5um,4.6×250mm)分离;流动相:0.19磷酸溶液-乙腈,梯度洗脱;流速:1.0ml ·min-1;柱温:40℃;检测波长:203nm。结果:人参皂甙Rg1、人参皂甙Re、人参皂甙Rb1分别在22.55451.3,111.72234.1,234.854697.2ug·ml-1内具有良好的线性关系;加标回收率分别为100.1%,101.0%,98.5%;RSD分别为2.65%,2.13%,1.98%(n=9)。结论:该方法简单易行,准确性和重复性好,可用于铁皮枫斗颗粒的质量控制。OBJECTIVE To determine the content of Ginsenoside Rgl, Ginsenoside Re and Ginsenoside Rbl in TiePiFengDou granules by HPLC. METHODS the sample was extracted by Water-saturated butanol and separated on a Ultimate AQ-C18 column(5um, 4.6×250mm). 0. 1% Phosphoric acid and acetonitrile was used as mobile phase with gradient elution at flow rate of 1.0 ml · min-1 The detection wavelength was set at 203 nm. RESULTS the linear ranges of Ginsenoside Rg 1, Ginsenoside Re and Ginsenoside Rb 1 were 22.55451.3, 111.72234.1 and 234.854697.2ug · m-1.And the average recoveries were 100.1%, 101.0% and 98.5% with the RSD of 2.65%,2.13%,1.98%(n=9),respectively. CONCLUSION This method is simple,rapid and with good repeatability,and can be used for the quality control of Tie PiFengDou Granules.

关 键 词：人参皂甙RB1 

分 类 号：R286.0[医药卫生—中药学]