基于DNA发夹结构的新型汞离子生物传感器  

A Novel Biosensor Based on DNA Hairpin Structure for Detection of Mercury Ion

作　　者：邓大庆[1,2] 蔡义林 王立梅[2] 朱颖越[2] 齐斌[2] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221000 [2]常熟理工学院生物与食品工程学院苏州食品生物技术重点实验室,江苏常熟215500

出　　处：《化学试剂》2015年第7期635-638,共4页Chemical Reagents

基　　金：江苏省基础研究计划(自然科学基金)项目(BK20130379);江苏省高校自然科学研究面上资助经费项目(13KJB550001);苏州市科技计划基金资助项目(SS201335)

摘　　要：基于T-Hg2＋-T特异性结合作用,利用实时荧光定量PCR技术的高灵敏性和高选择性,建立了测定汞离子的新方法。在最佳实验条件下,CT值与汞离子的浓度有很好的线性关系,其线性范围为5~150 nmol/L,检测限为3 nmol/L,其回收率为95%~107%,标准差〈5%。该方案可用于水样品中汞离子的检测。A novel method based on the specific binding of mercury in thymine-thymine mismatch complexes for detection of Hg2 ＋has been developed using the high sensitivity and high selectivity of real-time quantitative polymerase chain reaction technology.Under the optimum conditions,this method exhibited an excellent linear response between cycle threshold（ CT） and mercury ion concentration in the range of 5 ~ 150 nmol / L,and the limit detection was 3 nmol / L. The recoveries for the water samples were in the range of 95% ~ 107% and the relative standard deviation was less than 5%. This method can be used in the detection of mercury ion in water samples.

关 键 词：实时荧光定量PCR 汞离子 T-Hg2+-T 

分 类 号：O657[理学—分析化学]