陆地棉离层基因GhBOP1的克隆及其功能的初步分析  

Cloning of Abscission Zone Development Gene of Gossypiumhirsutum L.GhBOP1 and Preliminary Analysis of Its Function

作　　者：常璟[1,2] 晋昕[1,2] 庞金环[2] 司怀军[1] 张宁[1] 吴家和[1,2] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]中国科学院微生物研究所,北京100101

出　　处：《华北农学报》2015年第3期14-19,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然基金项目(31471544);棉花生物学国家重点实验室开放课题基金项目(CB2014B02)

摘　　要：为防止棉铃脱落提高结铃性,进而提高棉花产量,从陆地棉中分离出一个参与离层分化调控的相关基因GhBOP1。通过生物信息学分析,表明GhBOP1蛋白序列含有保守性强的BTB和ANK结构域;进化树分析显示该蛋白氨基酸序列与许多植物的同源蛋白相似程度很高,说明该类蛋白进化较慢。利用qRT-PCR进行组织特异性表达分析的结果表明,GhBOP1基因在根、茎、叶和花等组织器官中均有少量表达,但在离层中为优势表达,表达量为叶片表达水平的20倍。利用病毒诱导基因沉默(VIGS)技术干涉棉花GhBOP1基因的表达,同时进行低盐胁迫处理分析,对照植株叶片出现大量地脱落,而GhBOP1基因沉默植株叶片生长正常。总之GhBOP1基因在离层中优势表达,并对离层的形成可能具有调控功能。In this study,a GhBOP1 involved in abscission zone differentiation was examined in boll-shedding,and could be used as a new candidate gene for increasing cotton yield. Here,a cotton homolog of Arabidopsis BOP gene relating differentiation of abscission zone was isolated by PCR,which encoding protein contains BTB and ANK domains revealed by bioinformatics analysis. The phylogenetic tree analysis of GhBOP1 as an entry showed that the protein sequences from various plants have high identity,exhibiting slow evolution in amino acid changes. The results of qRT-PCR analysis showed that GhBOP1 gene predominantly expressed in abscission zone of petiole although it ubiquitously expressed in all organs,e. g. root,stem,leaf,and flower,etc. The expression level of GhBOP1 gene in abscission zone was 20-fold higher than that in leaf. Virus-induced gene silencing（ VIGS） technology was employed to repress GhBOP1 gene expression in this study. Under salt stress,VIGS plants kept leaves normally growing,however,the control plants exhibited leaf shedding. In short,these results primarily suggest that GhBOP1 gene is higher expression in abscission zone,and activated abscission zone formation. The molecular mechanism of this gene regulating differentiation and formation of abscission zone is deserved to further study.

关 键 词：棉花 离层 GhBOP1基因 病毒诱导基因沉默 

分 类 号：Q78[生物学—分子生物学]