绵羊PRLH及其受体基因的克隆及在下丘脑-垂体-性腺轴中差异表达的研究  

Cloning of ovine Prolactin Releasing Hormone and Its Receptor Gene and Their Different Expression in the HPG Axis

作　　者：赵强[1,2] 马友记[1,2] 李菁华[1,2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]甘肃省肉羊繁育生物技术工程实验室,甘肃民勤733300

出　　处：《华北农学报》2015年第3期20-24,共5页Acta Agriculturae Boreali-Sinica

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2012-19);国家科技支撑计划项目(2011BAD19B02-2);三区人才专项计划项目(GSAU-SQ-2014-03)

摘　　要：为了探讨PRLH及其受体基因在绵羊性腺轴中的表达,以绵羊下丘脑-垂体-性腺轴系为研究对象,克隆了PRLH及其受体基因PRLHR,构建了系统进化树,并利用qRT-PCR技术对PRLH及其受体基因在绵羊性腺轴中不同组织的表达差异做了研究。结果表明,绵羊PRLH和PRLHR mRNA核苷酸序列分别为113,237 bp,PRLH基因核苷酸序列与山羊、牛、人、小鼠的同源性分别为96.8%,93.3%,80.3%,74.6%,PRLHR基因与藏羚羊、山羊、牛、人和小鼠的同源性分别为100%,99.3%,97.3%,90.4%,84.4%;PRLH及其受体基因在绵羊的下丘脑、垂体、子宫和卵巢中均有一定量的表达,且PRLH基因在下丘脑、垂体和卵巢中的表达要显著低于子宫(P<0.05);PRLHR在子宫与垂体中的表达差异显著(P<0.05),其他差异不明显。说明下丘脑、垂体、子宫和卵巢是PRLH合成分泌和发挥作用的主要器官。To explore the expression of PRLH and its receptor gene in sheep gonadal axis,we successfully cloned the PRLH and receptor gene in organizations at the hypothalamic-pituitary-gonadal axis by used the sheep as experimental animal,got the phylogenetic tree. Also we used qRT-PCR technology to research different expression of PRLH and its receptor gene in different tissues at HPG axis. The results showed that sheep PRLH and PRLHR nucleotide sequences of mRNA were 113,237 bp. PRLH gene nucleotide sequence and goats,cattle,human,mouse homology were 96. 8%,93. 3%,80. 3%,74. 6%. PRLHR gene and the Tibetan antelope,goat,cattle,human and mouse homology were 100%,99. 3%,97. 3%,90. 4%,84. 4%. PRLH and its receptor gene in sheep hypothalamus,pituitary,uterine and ovary were expressed,and the expression of PRLH gene in hypothalamus,pituitary and ovary was significantly less than the uterine（ P〈0. 05）. Expression of PRLHR in uterus and pituitary were significantly different（ P〈0. 05）,no significant difference in other tissues（ P〈0. 05）. So the hypothalamus,pituitary,ovary and uterus were the main organ for the synthesis of secretion.

关 键 词：绵羊 PRLH PRLHR RT-PCR 差异表达 

分 类 号：Q78[生物学—分子生物学]