不同发育时间蓖麻鲜种子中毒蛋白含量的测定  

Measured at Different Developmental Time Content of Ricin from Fresh Castor Seed

作　　者：黄凤兰[1,2] 雷雪[1,3] 朱国立[1,2] 罗蕊[1] 陈晓凤[1] 赵永[1] 李跃[1] 孙华军[1] 

机构地区：[1]内蒙古民族大学生命科学院,内蒙古通辽028000 [2]内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽028000 [3]东北林业大学生命科学院,黑龙江哈尔滨150036

出　　处：《华北农学报》2015年第3期186-191,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30760123;31160290);国家农业部公益性行业专项(201003057);内蒙古自治区高等学校"青年科技英才支持计划"(NJYT-14-A10);市校合作项目(SXZD2012018);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(BMYJ2011009)

摘　　要：蓖麻是一种重要的油料作物,种子中含有毒蛋白,蓖麻毒蛋白具有相当明显的抗肿瘤作用。以2129蓖麻品系不同发育时间的鲜种子为材料,利用磷酸盐提取法、BCA试剂盒法和UVwin5紫外V5.1.0、Band Scan蛋白定量分析、Excel、IBM SPSS Statistics软件,研究种子中毒蛋白的动态积累过程。结果表明：不同发育时间蓖麻鲜种子中,毒蛋白的含量随着发育时间的增加而逐渐在发育完全成熟即60 d时达到最大;毒蛋白含量与粗蛋白含量、种仁质量、种子质量分别呈极显著正相关、显著正相关和正相关关系。基于种子中毒蛋白含量将蓖麻鲜种子的发育时间分为3类：第Ⅰ类包括30,40,50,60 d,这些发育时间蓖麻鲜种子中蓖麻毒蛋白含量较高;第Ⅱ类只包括10 d,毒蛋白含量较低;第Ⅲ类只包括20 d,毒蛋白含量中等。不同发育时间蓖麻鲜种子中毒蛋白的动态积累过程是,在种子发育10-20,20-30 d时迅速积累,30-60 d时积累速度较慢,完全成熟即60 d时毒蛋白含量达到最大。Castor is an important oil crops, seeds containing ricin,which have an quite obviously effect on anti-tumor. In this experiment,we used 2129 castor fresh seed which at different development time for the study,usingphosphate extraction method, BCA kit method, UVwin5, ultravioletV5. 1.0, BandSean protein quantitative analysisand Excel, IBM SPSS Statistics software to research dynamic accumulation process of ricin in seed. The results wereas follows ： ricin content of fresh castor seeds at different developmental time increases with development time, andgradually reached the maximum in 60 d when fully mature;ricin content and crude protein content was high-signifi-cant positive correlation, with quality of seeds was significant positive correlation, and seed quality were positive cor-relation. Based on seed ricin content fresh castor seed development time will be divided into three categories：Class I including 30,40,50,60 d, these castor seeds ricin content was higher, type II included only 10 d,low ricin con-tent ; class III included only 20 d, moderate riein. The conclusion is as follows ： dynamic accumulation process of ricinin fresh castor seeds at different developmental time is rapidly accumulated during seed development 10 -20,20 -30 d,then 30 -60 d became slow,ricin content reaches the maximum when 60 d fully ripe.

关 键 词：蓖麻种子 毒蛋白 发育时间 

分 类 号：S565.6[农业科学—作物学]