小麦河农6251苗期抗叶锈病基因的鉴定  

Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

作　　者：任晓娣[1] 杜冬冬[1] 张河山 姚宏鹏 赵赛[1] 杨文香[1,2] 刘大群[1,2,3] 

机构地区：[1]河北农业大学植物病理学系,河北保定071001 [2]河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [3]国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《华北农学报》2015年第3期192-199,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划项目(2013CB127700);河北省现代农业产业体系小麦产业创新团队建设项目

摘　　要：为明确河农6251含有的抗叶锈基因并对其进行分子定位,以抗病品种河农6251与感病品种Thatcher的杂种F1、F2、F3群体为材料,对河农6251的苗期抗叶锈基因进行定位。分子标记辅助鉴定结果表明,河农6251中含有抗叶锈基因Lr26和Lr ZH84,可能含有Lr2c和Lr17a;遗传分析表明,河农6251对叶锈菌PBGP的抗性由1对显性抗病基因决定,暂命名为Lr H6;用SSR技术和分离群体分组法(BSA)分析河农6251 F2群体和F3家系,结果位于1B染色体的3个SSR标记wmc419、wms582和barc120与Lr H6连锁,遗传距离分别为21.6,25.8,27.9 c M。河农6251中含有Lr26、Lr ZH84等多个抗叶锈基因,其对PBGP的抗性由一对位于1BL染色体上的显性抗叶锈基因决定。Preliminary research showed that they all contributed higher disease resistance level to Puccinia triticina. Detecting and mapping wheat leaf rust resistance gene were significance for application of the resistance in the wheat production. Resistant parents Henong 6251,susceptible parent Thatcher and their F2,F3 populations were employed to map the wheat leaf rust resistance genes in Henong 6251 based on SSR technique. Molecular marker-assisted identification results showed that Henong 6251 contained wheat leaf rust resistance genes Lr26 and Lr ZH84,and probably contained Lr2 c and Lr17 a. Henong 6251,Thatcher,and their Fl,F2 populations and F3 families were tested for detecting the wheat leaf rust resistance with single strain of Chinese P. triticina pathotype PBGP in greenhouse.The results showed that the wheat leaf rust resistance in Henong 6251 to PBGP was controlled by one dominant resistance gene,temporarily designated as Lr H6. SSR molecular markers and BSA were used to test Henong 6251 and their resistant and susceptible bulks of F2 and F3populations for mapping the leaf rust resistance gene. Results indicated that Henong 6251 carried a single dominant resistance gene Lr H6 to PBGP,and was preliminary located on chromosome 1B. The resistance gene Lr H6 was linked to the three known SSR loci（ wmc419,wms582 and barc120）on the 1BL with genetic distance ranging 21. 6,25. 8,27. 9 c M respectively. Henong 6251 contained more than one leaf rust resistance genes including Lr26 and Lr ZH84 and the wheat leaf rust resistance to PBGP was controlled by one dominant and new resistance gene.

关 键 词：小麦叶锈病 抗病基因 SSR标记 遗传分析 遗传作图 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]