全氟辛酸致小鼠脾脏氧化损伤的实验研究  

The experimental study of spleen oxidation damage induced by perfluorooctanoic acid in mice

作　　者：王力[1] 张梦媛[1] 吕卉[1] 申玲[1] 张玉媛[1] 赵文红[1] 

机构地区：[1]蚌埠医学院预防医学系,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2015年第6期716-718,共3页Journal of Bengbu Medical College

基　　金：安徽省自然科学研究项目(1508085QH188);蚌埠医学院省级特色专业"预防医学"专项(皖教高[2011]5号)

摘　　要：目的:探讨不同剂量全氟辛酸(perfluorooctanoic acid,PFOA)对小鼠脾脏组织的脂质过氧化损伤效应。方法:40只SPF级ICR小鼠,按单纯随机抽样方法分为正常对照组和低剂量组(1 mg/kg PFOA)、中剂量组(5 mg/kg PFOAS)、高剂量组(25mg/kg PFOA)3个染毒组,每组10只。染毒组各组每天1次灌胃相应剂量PFOA染毒,灌胃溶液体积为0.1 ml/10 g,正常对照组给予等体积0.9%氯化钠注射液,持续14 d,每天称质量1次,观察行为,14 d后称质量处死,计算脾脏系数,检测脾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、山梨醇脱氢酶(SDH)、乳酸脱氢酶(LDH)、丙二醛(MDA)及一氧化氮(NO)水平。结果:各染毒组小鼠出现不同程度的体质量增长缓慢甚至减轻,其中中、高剂量组出现明显减轻(P<0.01);各染毒组脾脏系数均明显高于对照组(P<0.05);与对照组相比,各剂量组的脾组织匀浆中MDA、NO及LDH含量明显增高,SOD、SDH及GSH-Px活性均明显降低(P<0.01)。结论:PFOA能抑制小鼠体质量的增长,对脾脏组织造成一定的脂质过氧化损伤。Objective：To investigate the effects of different doses of perfluorooctanoic acid（PFOA） on the spleen lipid peroxidation damage of mice. Methods：Forty SPF ICR mice were divided into the control group,and low（1 mg /kg）,medium（5 mg /kg） and high dose（25 mg /kg） PFOA groups（10 mice each group） using the simple random sampling method. All mice were administered by gavage for 14 days. The mice were weighed every day,and their behavior were observed. The spleen/body weight indexes were calculated after 14 days. The activities of superoxide dismutase （ SOD ） , glutathione peroxidase （ GSH-Px ） , sorbitol dehydrogenase （ SDH ） , lactate dehydrogenase（LDH）,and the levels of malondialdehyde（MDA）,nitric oxide（NO） were analyzed. Results：The body weight of mice treated with PFOA grew slowly or lightened,especially in middle and high dose group（P〈0. 01）. The spleen/body weight indexes of mice treated with PFOA were significantly lighter than that in control group（P〈0. 05）. Compared with control group,the levels of MDA NO and LDH increased, the activities of SOD, GSH-Px and SDH in mice treated with PFOA decreased obviously, respectively （ P 〈0. 01）. Conclusions：PFOA can inhibit the body weight growth of mice,and induce the spleen lipid peroxidation damage.

关 键 词：全氟辛酸 脾组织 氧化损伤 小鼠 

分 类 号：R284[医药卫生—中药学]