国槐花粉保存方法的初步研究  

A Preliminary Study on Sophora japonica Pollen Preservation Method

作　　者：牛燕娥 庞彩红[2,3] 李双云[2,3] 夏阳[2,3] 屈星[1] 

机构地区：[1]甘肃农业大学,兰州730000 [2]山东省林业科学研究院,济南250014 [3]山东省林木遗传改良重点实验室,济南250014

出　　处：《中国农学通报》2015年第19期96-100,共5页Chinese Agricultural Science Bulletin

基　　金：山东省农业良种工程"国槐等优质抗逆林草种质创新利用研究"(鲁科农字[2011]157号)

摘　　要：研究旨在对国槐花粉进行提早采集和保存,避免人工辅助授粉和杂交授粉带来的不便,延长花粉授粉期间的生活力。以国槐花粉为实验材料,使用培养基萌发法观察花粉萌发情况,测定花粉萌发所需时间,找出适于花粉萌发的培养基配方,同时比较花粉在室温(27℃左右)和冰箱冷藏室(4℃)2种保存方法下的寿命。结果表明:6种培养基中最适于花粉萌发的培养基为1 g琼脂+1 g蔗糖+100 mg/L硼酸;花粉的萌发时间为萌发60 min;保存相同天数的情况下,冰箱冷藏室花粉萌发率明显高于室温萌发率,冰箱冷藏室保存5天的花粉可用于国槐授粉,此时的花粉萌发率大于50%。实验获得了适于国槐花粉进行野外授粉的保存方法,旨在为研究槐属植物的杂交育种提供参考依据。The study aims at early acquisition and preservation of Sophora japonica pollen,avoidinginconvenience of artificial pollination and cross pollination,and prolonging the pollen viability during pollenpollination.Simple methods to evaluate the viability and to find out the time of pollen germination wereestablished.Pollen viability was assessed by improving a growing media and evaluating three common pollengermination media components： agar,sucrose,and boric acid.Meanwhile,the pollen in room temperature（about 27℃） and in the refrigerator freezer（4℃） were differentiated to determine their longevity.Resultsshowed that optimal germination of pollen was obtained at 1 g agar＋1 g sucrose＋100 mg/L boric acid.The timeof pollen germination was 60 minutes.Under the same number of preservation days,the pollen germination ratein refrigerator freezer was significantly higher than that in room temperature.The pollen stored 5 days inrefrigerator freezer was suitable for pollen pollination of Sophora japonica,and the pollen germination rate wasgreater than 50 percent.The suitable preservation method for field pollination of Sophora japonica pollen hasbeen obtained,which can provide a reference for research on hybridization breeding of Sophora plant.

关 键 词：国槐 花粉 培养基 萌发率 保存方法 

分 类 号：S792.26[农业科学—林木遗传育种]