X射线灭活活体日本沼虾精子的初步研究  

Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

作　　者：颜跃弟[1] 傅洪拓[1,2] 梁国霞 乔慧[2] 白鸿坤 蒋速飞[2] 熊贻伟[2] 龚永生[2] 金舒博[2] 张文宜[2] 吕洋[3] 吴滟[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海200000 [2]中国水产科学研究院淡水渔业研究中心/农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [3]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《中国农学通报》2015年第20期35-38,共4页Chinese Agricultural Science Bulletin

基　　金：江苏省科技支撑计划-农业部分"优质高产抗逆青虾新品种的选育"(BE2012334);国家"十二五"支撑"青虾新品种选育技术研究"(2012BAD2604-05);江苏省基础研究计划(自然科学基金)青年基金"青虾精巢发育调控基因的鉴别;克隆及表达研究"(BK2012091);江苏省三新工程工程项目"青虾新品种选育"(D2013-6);中央级公益性科研院所基本科研业务费专项资金项目"青虾性成熟相关基因的筛选及功能研究"(2015JBFM05)

摘　　要：为检测X射线对日本沼虾精子的灭活效果,利用X射线对雄性日本沼虾进行不同时间的照射处理,将处理后的雄虾与刚完成生殖脱壳的雌虾交配。待雌虾产卵后,计数雌虾抱卵天数,并在显微镜下观察卵的畸形情况。X射线管照射强度为100 k V,5 m A,源距10 cm,照射时间为0、30、60、90、120 min。结果发现：照射30 min组的抱卵天数及卵的畸形率较0 min组无变化,卵的畸形率为0,卵能正常发育至孵化出膜。随着照射时间的增加,在30-90 min之间,雌虾抱卵天数逐渐减少,且所抱卵的畸形率呈上升趋势。照射120 min组的抱卵天数较90 min组变化不大,卵的畸形率达100%。在本试验照射时间范围（0-120 min）内,灭活日本沼虾精子遗传物质的最佳照射时间为90-120 min。To investigate the effect of X-ray on sperm inactivation of Macrobrachium nipponense, male shrimpswere irradiated by 100 k V-5 m A X-ray for 0, 30, 60, 90, 120 min with a distance of 10 cm. Then the maleshrimps were made to mate with female soft-shelled shrimps. Broody days and aberration rate were recordedafter ovulation. The results showed that there was no difference between 0 min group and 30 min group, and theaberration rate was 0. Broody days gradually decreased while aberration rate increased with increasingirradiation time from 30 to 90 min. After 90 min, broody days showed no change, and the aberration ratereached a peak of 100%. In this research, the radiation time range was 0-120 min. According to the results,the optimum irradiation time to inactivate genetic material of sperms of Macrobrachium nipponense was 90-120 min.

关 键 词：日本沼虾 X射线 抱卵天数 畸形率 遗传物质 

分 类 号：S931[农业科学—渔业资源]