丛枝菌根真菌和外源水杨酸对番茄耐冷性的影响  

Effects of Arbuscular Mycorrhizal Fungus and Exogenous Salicylic Acid on Cold Tolerance of Tomato Plants

作　　者：陈丽平[1,2] 李敏[1,2] 

机构地区：[1]青岛农业大学菌根生物技术研究所,山东青岛266109 [2]青岛农业大学园艺学院

出　　处：《青岛农业大学学报（自然科学版）》2015年第2期79-82,91,共5页Journal of Qingdao Agricultural University(Natural Science)

基　　金："十二五"国家科技支撑计划项目(2014BAD05B03);山东省科技发展计划(2013GNC11014);青岛市基础研究项目(13-1-4-143-jch)

摘　　要：丛枝菌根真菌(AMF)和水杨酸(SA)能诱导植物抗逆性。本试验旨在温室条件下评价AMF和外源SA对番茄植株耐冷性的影响。试验设24个处理,将接种摩西球囊霉(Glomus mosseae)与不接种的番茄苗分别喷施0mg/L、200mg/L、400mg/L、600mg/L的SA,经5℃、10℃、15℃胁迫处理5d后,测定有关生理生化指标。结果表明,接种AMF、外施SA、或AMF+SA处理均能显著提高适温(昼/夜(25±0.5)℃/(15±0.5)℃)、低温(昼/夜(10±0.5)℃/(5±0.5)℃)和亚低温(昼/夜(15±0.5)℃/(10±0.5)℃)条件下番茄叶片叶绿素含量、降低丙二醛(MDA)含量、提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,其中以接种AMF+SA处理各项指标最佳。适温下AMF+SA 200mg/L处理叶片SOD、POD、CAT活性和叶绿素含量最高,MDA含量最低;低温和亚低温条件下AMF+SA 400mg/L处理叶片SOD、POD、CAT活性和叶绿素含量最高,MDA含量最低。结论认为,接种AMF配施400mg/L的外源SA能够显著提高番茄幼苗的耐冷性。Arbuscular mycorrhizal fungi（AMF）and salicylic acid（SA）can induce resistance of plants.The purpose of the present experiment was to estimate the effects of AMF and SA on cold-tolerance of tomato seedlings under greenhouse conditions.Total 24 treatments were designed with or without Glomus mosseae inoculation and added 0mg/L,200mg/L,400mg/L,and 600mg/L of SA on tomato seedlings,then to determine physiological and biochemical parameters 5dafter treatment under 5 ℃、10 ℃ and 15 ℃ conditions.Results showed that AMF,exogenous SA,or AMF＋SA treatment could significantly increase the chlorophyll content,reduce malondialdehyde（MDA）content,and increase superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT）activity in the leaves of tomato plants under the normal temperature（day/night（25±0.5）℃/（15±0.5）℃）,low temperature（day/night（10±0.5）℃/（5±0.5）℃）and sub-hypothermia（day/night（15±0.5）℃/（10±0.5）℃）.AMF＋SA showed the best benefit effects.AMF＋SA 200mg/L treatment gave the highest SOD,POD,CAT activity and chlorophyll contents,and the lowest MDA content in the leaves of tomato plants under normal temperature;while AMF＋SA 400mg/L treatment gave the highest SOD,POD,CAT activity and chlorophyll content,and the minimum MDA content under the lower temperatures and sub-hypothermia.It is concluded that G.mosseaeinoculation with adding 400mg/L of exogenous SA can significantly improve the cold tolerance of tomato seedlings.

关 键 词：水杨酸 AM真菌 番茄 抗冷性 

分 类 号：Q943[生物学—植物学] S641.2[农业科学—蔬菜学]