酸浆苦素B的体外抗肿瘤活性研究  

Study on Anti-tumor Activities of Physalins B in Vitro

作　　者：方春生[1] 杨燕军[1] 

机构地区：[1]广东食品药品职业学院,广东广州510520

出　　处：《广州中医药大学学报》2015年第4期652-655,660,782,783,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2011B031700072);广东省自然科学基金项目(编号:S2013010013484;9151063201000036);广东省中医药管理局重点项目(编号:2008435);广东省中医药管理局项目(编号:20141202);广东食品药品学院院级项目(编号:2013YZ008)

摘　　要：【目的】研究苦蘵中提取的酸浆苦素B(physalin B,Ph B)的体外抗肿瘤活性。【方法】采用四甲基偶氮唑蓝(MTT)比色法和细胞形态观察法研究Ph B对2种肿瘤细胞株Hep G2和SGC7901增殖的抑制作用及形态的影响,并与抗癌药物羟基喜树碱(hydroxycamptothecine,HCTP)作对照比较;采用4’6-二脒基-2-苯基吲哚(DAPI)染色法观察Ph B诱导Hep G2和SGC7901细胞凋亡。【结果】MTT实验结果显示Ph B对Hep G2和SGC7901的生长均有明显的抑制作用,并具有一定的时间剂量依赖关系,且高浓度时作用优于阳性对照药HCTP,倒置相差显微镜结果也显示其显著改变了细胞形态,并能够证实MTT实验结果的可靠性;DAPI染色结果表明Ph B可促进Hep G2和SGC7901细胞凋亡。【结论】苦蘵提取物Ph B对肿瘤细胞Hep G2和SGC7901具有一定的增殖抑制作用。Objective To study the in-vitro anti-tumor activities of physalin B（Ph B） extracted from Physalis angulata L.. Methods The effect of Ph B on the cell proliferation and the morphology changes of tumor cell strains Hep G2 and SGC7901 was observed by methyl thiazolyl tetrazolium（MTT） assay and by phase contrast microscopy respectively, and the results were compared to positive control anti-cancer drug hydroxycamptothecine（HCTP）.Apoptosis of Hep G2 and SGC7901 cells induced by Ph B was determined by 4＇, 6-diamidino-2-phenylindole（DAPI） staining method. Results MTT results showed that the growth of Hep G2 and SGC7901 was significantly inhibited by Ph B in dose-and time-dependent manner, and the inhibitory effect of high concentration of Ph B was better than the positive control medicine HCTP. Phase contrast microscopy results showed that the cellular morphology was improved by Ph B, which was accorded with the results showed by MTT assay. DAPI staining results showed that the apoptosis of Hep G2 and SGC7901 was enhanced by Ph B. Conclusion Ph B extracted from Physalis pubescens L. exerts certain inhibitory effect on the proliferation of Hep G2 and SGC7901 cells.

关 键 词：酸浆苦素B/药理学 肿瘤/中药疗法 肿瘤细胞/病理学 细胞增殖 细胞凋亡 细胞培养 

分 类 号：R285.5[医药卫生—中药学]