针刺对胰岛素抵抗模型大鼠骨骼肌胰岛素受体底物和葡萄糖转运蛋白4的影响  

Effect of Acupuncture on Insulin Receptor Substrate and Glucose Transporter 4 in Skeletal Muscle of Experimental Rats with Insulin Resistance

作　　者：张梁[1] 易玮[1] 

机构地区：[1]广州中医药大学针灸康复临床医学院,广东广州510405

出　　处：《广州中医药大学学报》2015年第4期699-704,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30873294;81273845;81303043)

摘　　要：【目的】观察针刺对胰岛素抵抗模型大鼠骨骼肌胰岛素受体底物-1(IRS-1)、胰岛素受体底物-2(IRS-2)和葡萄糖转运蛋白4(GLUT4)的影响。【方法】将32只SD大鼠随机分成正常组、正常针刺组、模型组和模型针刺组,采用葡萄糖氧化酶法、酶联免疫吸附法(ELISA)、实时荧光定量PCR等方法,检测并比较各组大鼠空腹血糖(FPG),血浆胰岛素(FINS),胰岛素敏感性指数(ISI),血清C-肽(C-P),骨骼肌IRS-1、IRS-2、GLUT4 m RNA表达。【结果】模型组大鼠的FPG、FINS、C-P水平较正常组显著升高(P<0.01),ISI,IRS-1、IRS-2、GLUT4 m RNA表达较正常组显著降低(P<0.01);模型针刺组的FPG、FINS、C-P水平较模型组显著降低(P<0.05或P<0.01),ISI,IRS-1、IRS-2、GLUT4 m RNA表达较模型组显著升高(P<0.01)。【结论】针刺可能在基因转录水平对IRS-1、IRS-2、GLUT4产生影响,从而改善PI3K通路的信号转导。Objective To observe the curative effect of acupuncture on insulin receptor substrate 1（IRS-1）,IRS-2 and glucose transporter 4（GLUT4） in skeletal muscle of experimental rats with insulin resistance（IR）.Methods Thirty-two rats were randomly divided into normal group, model group, acupuncture normal group and acupuncture model group. The fasting plasma glucose（FPG） level was detected with glucose oxidase method, fasting insulin and C-peptide（C-P） were examined with enzyme linked immunosorbent assay（ELISA）, insulin sensitivity index（ISI） was calculated, and IRS-1 m RNA, IRS-2 m RNA and GLUT4 m RNA of skeletal muscle were observed by real-time PCR-based fluorescent assay in each group. Results Compared to the normal group, the FPG, FINS, C-P levels were increased dramatically（P〈0.01）, ISI, IRS-1m RNA, IRS-2 m RNA and GLUT4 m RNA of the model group declined remarkably（P〈0.01）. Compared to the model group, the FPG, FINS, C-P levels declined evidently（P〈0.05 or P〈0.01）, and ISI, IRS-1 m RNA,IRS-2 m RNA and GLUT4 m RNA of the acupuncture model group were increased dramatically（P〈0.01）.Conclusion The curative mechanism of acupuncture for insulin resistance is probably related to the increase of IRS-1 m RNA, IRS-2 m RNA and GLUT4 m RNA expression in skeletal muscle which contributes to the improvement of PI3 K signal pathway.

关 键 词：胰岛素抵抗/针灸疗法 基因表达调控 疾病模型 动物 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]