熟地寄生壮骨方对膝骨关节炎模型大鼠关节软骨形态的影响  

Effect of Shudi Jisheng Zhuanggu Decoction on Morphology of Articular Cartilage of Knee Osteoarthritis Model Rats

作　　者：周颖燕[1] 徐侦雄[2] 林洁华[1] 何晓红[2] 陈更新[3] 陈玉兴 赵自明 何羿婷[3] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510405 [2]广东省中医院风湿科,广东广州510120 [3]广东省中医院新药开发办,广东广州510120 [4]广东省中医药科学院,广东广州510120

出　　处：《广州中医药大学学报》2015年第4期711-714,784,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技厅基金资助项目(编号:2011B032200016)

摘　　要：【目的】观察熟地寄生壮骨方对膝骨关节炎模型大鼠关节软骨形态的影响。【方法】选用SD大鼠,随机分为7组,即空白对照组,模型对照组,必理通片组(剂量为0.270 g/kg),壮骨关节丸组(剂量为1.08 g/kg),熟地寄生壮骨方高、中、低剂量组(剂量分别为27.450、13.725、6.863 g/kg);采用关节腔注射木瓜蛋白酶法复制右膝骨关节炎模型,测量给药前后各组大鼠膝骨关节厚度,观察各组对右膝骨关节炎模型的影响;取右膝关节软骨及软骨下骨,制成组织切片后进行苏木精—伊红(HE)染色,采用Mankin软骨组织学评分法进行评分。【结果】与空白对照组比较,模型组膝骨关节肿胀度显著增大(P<0.01),Mankin评分值显著增加(P<0.01);与模型对照组比较,熟地寄生壮骨方各剂量组大鼠膝骨关节肿胀度显著减小(P<0.05或P<0.01),Mankin评分值均显著降低(P<0.01),膝关节软骨病理变化均减轻。【结论】熟地寄生壮骨方能改善膝骨关节炎模型大鼠膝关节软骨形态,达到改善软骨退变、保护软骨的目的。Objective To observe the morphology changes in articular cartilage of knee osteoarthritis （KOA） model rats after intervention with Shudi Jisheng Zhucmggu Decoction （SJZD）. Methods SD rats were randomized into 7 groups, namely blank control group, model group, Paracetamol group （0.270 g/kg）, Zhuangu Guanjie Pills group （1.08 g/kg）, and high-, middle- and low-dosage SJZD groups （27.450, 13.725, 6.863 g/kg, respectively）. Rats were injected with papain solution into the right knee joint to build models of KOA before administration of drugs. Thickness of right knee joint was measured to evaluate the effect of SJZD on the swelling degree of knee joint. Histological changes of knee cartilage were evaluated by Mankin scoring method after the cartilage sections being stained with hematoxylin-eosin （HE）. Results Swelling degree of knee joint and Mankin scores were significantly increased in the model group compared to the blank control group （P〈0.01）. In comparison with the model group, the swelling degree of knee joint and Mankin scores of SJZD groups were significantly decreased （P〈0.05 or P〈0.01）, and the pathological changes were relieved. Conclusion SJZD can ameliorate the pathological changes of knee articular cartilage in rats with KOA, so as to delay cartilage degeneration and protect the cartilage.

关 键 词：膝骨关节炎/中药疗法 熟地寄生壮骨方/药理学 软骨/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]