通窍化瘀法对大鼠视网膜血管内皮和视神经放射性损伤的预防作用  

Prevention of Retinal Vascular Endothelium and Radiation-induced Rat Optic Nerve Damage with Orifices-dredging and Stasis-removing Therapy

作　　者：冀建平[1] 程先华[2] 廉丽华[1] 刘红[1] 陈蕾[1] 李志英[1] 

机构地区：[1]广州中医药大学第一附属医院眼科,广东广州510405 [2]南昌大学眼科医院,江西南昌330031

出　　处：《广州中医药大学学报》2015年第4期715-719,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2009B060700071)

摘　　要：【目的】探讨通窍化瘀法对大鼠视网膜血管内皮细胞和视神经放射性损伤的预防作用。【方法】选择SD大鼠36只,随机分为空白组、模型组和实验组,模型组与实验组均采用放射线照射3周,每周1次;实验组于放射照射前2周开始每日腹腔内注射黄芪注射液(剂量为4.0 g/kg)、盐酸川芎嗪注射液(剂量为15 mg/kg)和醒脑静注射液(剂量为2.5 m L/kg),空白组和模型组每日腹腔注射等量生理盐水。采用酶联免疫吸附(ELISA)法测定各组放射照射前、照射后1 d、2周、4周及6周大鼠静脉血内皮素-1(ET-1)和血管性血友病因子(v WF)的浓度,分析其动态变化和组间差异。于照射后2、4、6周分别取材进行视神经透射电镜检查,观察视神经轴索脱髓鞘病变程度,并计算和比较各组视神经脱髓鞘率。【结果】与空白组比较,放射照射后模型组静脉血ET-1、v WF浓度显著升高,差异均有统计学意义(P<0.05),并于照射后4周达到峰值;与模型组比较,照射2周后实验组静脉血ET-1、v WF浓度显著降低,差异均有统计学意义(P<0.05);与空白组比较,放射照射后2、4、6周模型组视神经脱髓鞘率显著增高,差异均有统计学意义(P<0.05);模型组和实验组比较视神经脱髓鞘率在照射后2、4周差异均无统计学意义(P>0.05),照射后6周实验组视神经脱髓鞘率显著低于模型组,差异有统计学意义(P<0.05)。【结论】通窍化瘀法预防性干预能够显著降低放射性视网膜视神经损伤模型大鼠静脉血ET-1、v WF浓度,减轻放射线对视网膜血管内皮的损伤,并对放射性视神经损伤后期轴索脱髓鞘改变有改善作用。Objective To evaluate the preventive effect of orifices-dredging and stasis-removing therapy on retinal vascular endothelium and optic nerve damage induced by radiation in rats. Methods Thirty-six SD rats were randomly divided into 3 groups, namely blank control group, model group and therapy group. Rats in the model group and therapy group were exposed to X-ray radiation once a week for three weeks. Two weeks before the radiation,therapy group was pretreated with intraperitoneal injection of Astragalus injection（4.0 g/kg）, Ligustrazine Hydrochloride injection（15 mg/kg） and Xingnaojing injection（2.5 m L/kg） once a day, blank control group and model group were given intraperitoneal injection of same volume of saline once a day. Before radiation, one day, and 2, 4 and 6 weeks after radiation, we detected the blood concentrations of endothelin-1（ET-1） and Von willebrand factor（v WF） in all of the rats by enzyme-linked immunosorbent assay（ELISA）, and analyzed the dynamic changes and intergroup difference. Demyelination of optic nerve was observed under transmission electron microscope, and demyelination percentage was counted 2, 4, and 6 weeks after irradiation. Results Compared with the blank control group, the blood concentrations of ET-1 and v WF in model group were increased after irradiation（P〈0.05）, and the peak value appeared on the fourth week after irradiation. Compared with the model group, the blood concentrations of ET-1 and v WF in the therapy group were decreased weeks 2 after irradiation（P〈0.05）.The demyelination percentage of optic nerve was significantly higher in the model group than that in the blank control group（P〈0.05） 2, 4, and 6 weeks after irradiation. Compared with the model group, the demyelination percentage of optic nerve in therapy group showed insignificant changes 2 and 4 weeks after irradiation（P〉0.05）,but was decreased obviously 6 weeks after irradiation（P〈0.05）. Conclusion Pretreatment with orifices-dredging and stasis-removing t

关 键 词：放射性视神经病变/中药疗法 通窍化瘀法 视神经/超微结构 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]