不同剂量地塞米松对小鼠胸腺细胞凋亡的影响  

Effects of dexamethasone with different doses on appotosis of mouse thymocytes

作　　者：万书丽 庞鑫 东野广智[3] 郑贺[1] 王燕[1] 何其励 张芳成 孟庆勇[1] 

机构地区：[1]广东医学院检验医学研究所,广东东莞523808 [2]河南省南阳南石医院,河南南阳473000 [3]广东医学院分析中心,广东湛江524023

出　　处：《广东医学院学报》2015年第1期35-38,44,共5页Journal of Guangdong Medical College

基　　金：国家自然科学基金资助项目(No.81072238)

摘　　要：目的探讨地塞米松诱导小鼠胸腺细胞凋亡的适宜剂量。方法小鼠腹腔注射不同剂量(20、40、50mg/kg)地塞米松后12 h处死,提取胸腺细胞DNA,用琼脂糖凝胶电泳方法观察DNA梯形条带(DNA ladder),再用流式细胞术和电镜检测胸腺细胞凋亡情况。结果 40、50 mg/kg地塞米松组出现DNA ladder,流式细胞术发现凋亡峰,电镜观察显示凋亡小体。结论 40 mg/kg地塞米松可诱导小鼠胸腺细胞凋亡。Objective To study the optimal dose of dexamethasone that induces apoptosis of mouse thymocytes. MethodsThe mice were sacrificed 12 h after intraperitoneal injection with different doses（20, 40 and 50 mg/kg） of dexamethasone. DNA ladder and apoptosis of thymocytes were determined by agarose gel electrophoresis, flow cytometry and electron microscopy, respectively. Results DNA ladder and apoptotic peak and bodies were only present after treatment with 40 mg/kg or 50 mg/kg dexamethasone. Conclusion Dexamethasone of 40 mg/kg can induce apoptosis of mouse thymocytes.

关 键 词：小鼠 胸腺细胞 地塞米松 凋亡 

分 类 号：R392.12[医药卫生—免疫学]