三种银杏基因组DNA提取方法的比较  

Comparison of three extraction methods of genomic DNA of Ginkgo biloba L.

作　　者：钱卫东[1] 周颖欣[1] 龚国利[1] 蔡长龙[2] 吴启航[1] 张雨 

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]西安工业大学光电工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2015年第4期117-120,共4页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金青年项目(3100040);西安工业大学陕西省薄膜技术与光学检测重点实验室开放基金项目(ZSKJ201405)

摘　　要：为获得银杏基因组DNA的最佳提取方法,本文以银杏嫩叶为研究对象,利用传统CTAB法、改良CTAB法、改良SDS法等方法提取银杏基因组DNA,进而利用凝胶电泳法、紫外分光光度法、PCR扩增等方法分析提取效率和质量,确定最适合银杏基因组DNA的提取方法.结果表明,改良CTAB法对银杏基因组DNA提取效果最佳,为3种方法中最适合提取银杏基因组DNA的方法.In order to obtain a suitable extraction method of genomic DNA fromGinkgo biloba leaves,here the method of traditional CTAB,improved CTAB method,improved SDS method were investigated to extract genomic DNA of Ginkgobiloba.To assess the quality of DNA extraction,gel electrophoresis,UV spectrophotometry and PCR amplification were used to analyze the genomic DNA of GinkgobilobaL.The results show that the quantity and the quality of the DNA extracted by the improved CTAB is high enough to perform PCR hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques.

关 键 词：银杏 基因组DNA 提取 改良CTAB法 

分 类 号：R285.5[医药卫生—中药学]