盐酸曲马多口服控释片的高效液相色谱测定方法研究  

Study on the HPLC determination method of tramadol hydrochloride oral controlled-release tablet

作　　者：王兰[1] 李雅明[1] 程刚[1] 常相娜[1] 姬海红[2] 

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]成都大学研究生处,四川成都610106

出　　处：《陕西科技大学学报（自然科学版）》2015年第4期127-130,135,共5页Journal of Shaanxi University of Science & Technology

基　　金：西安市未央区科技计划项目(201209)

摘　　要：建立了高效液相色谱法（HPLC）测定盐酸曲马多口服控释片中盐酸曲马多的含量.采用十八烷基硅烷键合硅胶填充柱（C18柱）为色谱柱,流动相为[醋酸-醋酸钠缓冲液（pH4.5）]-甲醇（65∶35）,流速为1.0mL/min,柱温为30℃,检测波长271nm,进样量为20μl.样品与对照品出峰时间一致,TR≈8.0min;并且在0.3~0.7mg/mL浓度范围内线性关系良好,回收率达98%~102%.高效液相色谱法测定盐酸曲马多控释片,简单易行、重复性好.The objective of the present study was to establish a high performance liquid chromatography（HPLC）determination method of tramadol hydrochloride controlled release tablet.An octadecylsilane bonded silica gel packed column（C18column）was used as the chromatographic column.The mobile phase was[acetic acid-sodium acetate buffer（pH4.5）]-methanol（65∶35）,and the flow rate was 1.0mL/min.The column temperature was 30 ℃,the detection wavelength was 271 nm,and the injection volume was 20μl.A consistent peak time between the control and sample was achieved（TR≈8.0min）.In the concentration range of0.3~0.7mg/mL,the linearity was good.The recovery was measured as 98%~102%.Results of this work showed that the HPLC determination method of tramadol hydrochloride controlled-release tablet was simple and repeatable.

关 键 词：盐酸曲马多 控释片 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]